Leptin Levels Are Higher in Whole Compared to Skim Human Milk, Supporting a Cellular Contribution
AbstractHuman milk (HM) contains a plethora of metabolic hormones, including leptin, which is thought to participate in the regulation of the appetite of the developing infant. Leptin in HM is derived from a combination of de novo mammary synthesis and transfer from the maternal serum. Moreover, leptin is partially lipophilic and is also present in HM cells. However, leptin has predominately been measured in skim HM, which contains neither fat nor cells. We optimised an enzyme-linked immunosorbent assay for leptin measurement in both whole and skim HM and compared leptin levels between both HM preparations collected from 61 lactating mothers. Whole HM leptin ranged from 0.2 to 1.47 ng/mL, whilst skim HM leptin ranged from 0.19 to 0.9 ng/mL. Whole HM contained, on average, 0.24 ± 0.01 ng/mL more leptin than skim HM (p < 0.0001, n = 287). No association was found between whole HM leptin and fat content (p = 0.17, n = 287), supporting a cellular contribution to HM leptin. No difference was found between pre- and post-feed samples (whole HM: p = 0.29, skim HM: p = 0.89). These findings highlight the importance of optimising HM leptin measurement and assaying it in whole HM to accurately examine the amount of leptin received by the infant during breastfeeding. View Full-Text
A printed edition of this Special Issue is available here.
Share & Cite This Article
Kugananthan, S.; Lai, C.T.; Gridneva, Z.; Mark, P.J.; Geddes, D.T.; Kakulas, F. Leptin Levels Are Higher in Whole Compared to Skim Human Milk, Supporting a Cellular Contribution. Nutrients 2016, 8, 711.
Kugananthan S, Lai CT, Gridneva Z, Mark PJ, Geddes DT, Kakulas F. Leptin Levels Are Higher in Whole Compared to Skim Human Milk, Supporting a Cellular Contribution. Nutrients. 2016; 8(11):711.Chicago/Turabian Style
Kugananthan, Sambavi; Lai, Ching T.; Gridneva, Zoya; Mark, Peter J.; Geddes, Donna T.; Kakulas, Foteini. 2016. "Leptin Levels Are Higher in Whole Compared to Skim Human Milk, Supporting a Cellular Contribution." Nutrients 8, no. 11: 711.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.