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Open AccessArticle

Dietary Selenium Levels Affect Selenoprotein Expression and Support the Interferon-γ and IL-6 Immune Response Pathways in Mice

Department of Biological Sciences, Towson University, Towson, MD, 21252, USA
Molecular Biology of Selenium Section, Mouse Cancer Genetics Program, Center for Cancer Research, National Institutes of Health, Bethesda, MD, 20892, USA
Human Genetics, University of Utah, Salt Lake City, UT, 84112, USA
Nutritional Science Research Group, National Cancer Institute, Rockville, MD, 20892, USA
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Nutrients 2015, 7(8), 6529-6549;
Received: 23 June 2015 / Revised: 23 July 2015 / Accepted: 28 July 2015 / Published: 6 August 2015
(This article belongs to the Special Issue Dietary Selenium and Human Health)
Selenium is an essential element that is required to support a number of cellular functions and biochemical pathways. The objective of this study was to examine the effects of reduced dietary selenium levels on gene expression to assess changes in expression of non-selenoprotein genes that may contribute to the physiological consequences of selenium deficiency. Mice were fed diets that were either deficient in selenium or supplemented with selenium in the form of sodium selenite for six weeks. Differences in liver mRNA expression and translation were measured using a combination of ribosome profiling, RNA-Seq, microarrays, and qPCR. Expression levels and translation of mRNAs encoding stress-related selenoproteins were shown to be up-regulated by increased selenium status, as were genes involved in inflammation and response to interferon-γ. Changes in serum cytokine levels were measured which confirmed that interferon-γ, as well as IL-6, were increased in selenium adequate mice. Finally, microarray and qPCR analysis of lung tissue demonstrated that the selenium effects on immune function are not limited to liver. These data are consistent with previous reports indicating that adequate selenium levels can support beneficial immune responses, and further identify the IL-6 and interferon-γ pathways as being responsive to dietary selenium intake. View Full-Text
Keywords: IL-6; interferon-γ; ribosome profiling; selenium; selenocysteine; selenoprotein IL-6; interferon-γ; ribosome profiling; selenium; selenocysteine; selenoprotein
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Tsuji, P.A.; Carlson, B.A.; Anderson, C.B.; Seifried, H.E.; Hatfield, D.L.; Howard, M.T. Dietary Selenium Levels Affect Selenoprotein Expression and Support the Interferon-γ and IL-6 Immune Response Pathways in Mice. Nutrients 2015, 7, 6529-6549.

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