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Nutrients 2015, 7(8), 6425-6445;

Folate Deficiency Could Restrain Decidual Angiogenesis in Pregnant Mice

Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Chongqing 400016, China
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 27 March 2015 / Revised: 27 March 2015 / Accepted: 20 July 2015 / Published: 4 August 2015
(This article belongs to the Special Issue Nutrition in Pregnancy)
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The mechanism of birth defects induced by folate deficiency was focused on mainly in fetal development. Little is known about the effect of folate deficiency on the maternal uterus, especially on decidual angiogenesis after implantation which establishes vessel networks to support embryo development. The aim of this study was to investigate the effects of folate deficiency on decidual angiogenesis. Serum folate levels were measured by electrochemiluminescence. The status of decidual angiogenesis was examined by cluster designation 34 (CD34) immunohistochemistry and the expression of angiogenic factors, including vascular endothelial growth factor A (VEGFA), placental growth factor (PLGF), and VEGF receptor 2 (VEGFR2) were also tested. Serum levels of homocysteine (Hcy), follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), progesterone (P4), and estradiol (E2) were detected by Enzyme-linked immunosorbent assay. The folate-deficient mice had a lower folate level and a higher Hcy level. Folate deficiency restrained decidual angiogenesis with significant abnormalities in vascular density and the enlargement and elongation of the vascular sinus. It also showed a reduction in the expressions of VEGFA, VEGFR2, and PLGF. In addition, the serum levels of P4, E2, LH, and PRL were reduced in folate-deficient mice, and the expression of progesterone receptor (PR) and estrogen receptor α (ERα) were abnormal. These results indicated that folate deficiency could impaire decidual angiogenesis and it may be related to the vasculotoxic properties of Hcy and the imbalance of the reproductive hormone. View Full-Text
Keywords: folate deficiency; decidual angiogenesis; reproductive hormone folate deficiency; decidual angiogenesis; reproductive hormone

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Li, Y.; Gao, R.; Liu, X.; Chen, X.; Liao, X.; Geng, Y.; Ding, Y.; Wang, Y.; He, J. Folate Deficiency Could Restrain Decidual Angiogenesis in Pregnant Mice. Nutrients 2015, 7, 6425-6445.

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