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Open AccessArticle

Proteomics Analysis to Identify and Characterize the Molecular Signatures of Hepatic Steatosis in Ovariectomized Rats as a Model of Postmenopausal Status

Proteomics Research Center, National Yang-Ming University, Taipei 11221, Taiwan
Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 33301, Taiwan
Department of Orthopedic Surgery, Taipei Medical University Shuang Ho Hospital, New Taipei City 23561, Taiwan
School of Nutrition and Health Sciences, Taipei Medical University, Taipei 11031, Taiwan
National Laboratory Animal Center, National Applied Research Laboratories, Taipei 11529, Taiwan
Department of Forestry, National Chung Hsing University, Taichung 40227, Taiwan
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Nutrients 2015, 7(10), 8752-8766;
Received: 28 August 2015 / Revised: 7 October 2015 / Accepted: 15 October 2015 / Published: 22 October 2015
Postmenopausal women are particularly at increased risk of developing non-alcoholic fatty liver disease (NAFLD). Here we aimed to determine the impact of postmenopausal-induced NAFLD (PM-NAFLD) in an ovariectomized rat model. Sixteen six-week-old Sprague-Dawley female rats were randomly divided into two groups (eight per group), for sham-operation (Sham) or bilateral ovariectomy (Ovx). Four months after surgery, indices of liver damage and liver histomorphometry were measured. Both serum aspartate aminotransferase (AST) and alanine aminotranferease (ALT) levels were significantly higher in the Ovx than Sham group. We performed quantitative LC-MS/MS-based proteomic profiling of livers from rats with PM-NAFLD to provide baseline knowledge of the PM-NAFLD proteome and to investigate proteins involved in PM-NAFLD by ingenuity pathways analysis (IPA) to provide corroborative evidence for differential regulation of molecular and cellular functions affecting metabolic processes. Of the 586 identified proteins, the levels of 59 (10.0%) and 48 (8.2%) were significantly higher and lower, respectively, in the Ovx group compared to the Sham group. In conclusion, the changes in regulation of proteins implicated in PM-NAFLD may affect other vital biological processes in the body apart from causing postmenopause-mediated liver dysfunction. Our quantitative proteomics analysis may also suggest potential biomarkers and further clinical applications for PM-NAFLD. View Full-Text
Keywords: liver; proteome; ingenuity pathways analysis liver; proteome; ingenuity pathways analysis
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Liao, C.-C.; Chiu, Y.-S.; Chiu, W.-C.; Tung, Y.-T.; Chuang, H.-L.; Wu, J.-H.; Huang, C.-C. Proteomics Analysis to Identify and Characterize the Molecular Signatures of Hepatic Steatosis in Ovariectomized Rats as a Model of Postmenopausal Status. Nutrients 2015, 7, 8752-8766.

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