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Open AccessArticle

A Novel Role of Eruca sativa Mill. (Rocket) Extract: Antiplatelet (NF-κB Inhibition) and Antithrombotic Activities

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Department of Clinical Biochemistry and Immunohematology, Faculty of Health Sciences, Interdisciplinary Excellence Research Program on Healthy Aging (PIEI-ES), Universidad de Talca, Talca 3460000, Chile
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Centro de Estudios en Alimentos Procesados (CEAP), CONICYT-Regional, Gore Maule 3460000, R09I2001, Chile
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Horticulture Department, Faculty of Agricultural Sciences, Universidad de Talca, Talca 3460000, Chile
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Authors to whom correspondence should be addressed.
Nutrients 2014, 6(12), 5839-5852; https://doi.org/10.3390/nu6125839
Received: 29 July 2014 / Revised: 24 November 2014 / Accepted: 25 November 2014 / Published: 12 December 2014
Background: Epidemiological studies have shown the prevention of cardiovascular diseases through the regular consumption of vegetables. Eruca sativa Mill., commonly known as rocket, is a leafy vegetable that has anti-inflammatory activity. However, its antiplatelet and antithrombotic activities have not been described. Methods: Eruca sativa Mill. aqueous extract (0.1 to 1 mg/mL), was evaluated on human platelets: (i) P-selectin expression by flow cytometry; (ii) platelet aggregation induced by ADP, collagen and arachidonic acid; (iii) IL-1β, TGF-β1, CCL5 and thromboxane B2 release; and (iv) activation of NF-κB and PKA by western blot. Furthermore, (v) antithrombotic activity (200 mg/kg) and (vi) bleeding time in murine models were evaluated. Results: Eruca sativa Mill. aqueous extract (0.1 to 1 mg/mL) inhibited P-selectin expression and platelet aggregation induced by ADP. The release of platelet inflammatory mediators (IL-1β, TGF-β1, CCL5 and thromboxane B2) induced by ADP was inhibited by Eruca sativa Mill. aqueous extract. Furthermore, Eruca sativa Mill. aqueous extract inhibited NF-κB activation. Finally, in murine models, Eruca sativa Mill. aqueous extract showed significant antithrombotic activity and a slight effect on bleeding time. Conclusion: Eruca sativa Mill. presents antiplatelet and antithrombotic activity. View Full-Text
Keywords: Eruca sativa Mill.; rocket; antiplatelet; antithrombotic; platelet inflammatory mediators; functional food Eruca sativa Mill.; rocket; antiplatelet; antithrombotic; platelet inflammatory mediators; functional food
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Fuentes, E.; Alarcón, M.; Fuentes, M.; Carrasco, G.; Palomo, I. A Novel Role of Eruca sativa Mill. (Rocket) Extract: Antiplatelet (NF-κB Inhibition) and Antithrombotic Activities. Nutrients 2014, 6, 5839-5852.

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