Pathology of Bursae of Fabricius in Methionine-Deficient Broiler Chickens
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chickens and Diets
2.2. Clinical Signs and Relative Bursa Weights
2.3. Pathological Observation
2.4. Cell Cycle of Bursa of Fabricius
2.5. Annexin-V Apoptosis Detection by Flow Cytometry
2.6. Statistical Analysis
3. Results
3.1. Clinical Observation
Groups | 7 days | 14 days | 21 days | 28 days | 35 days | 42 days |
---|---|---|---|---|---|---|
Control group | 148.40 ± 3.05 | 354.27 ± 9.24 | 657.73 ± 20.11 | 999.80 ± 28.16 | 1347.40 ± 54.42 | 1781.33 ± 78.18 |
Met Deficiency | 138.40 ± 2.38 | 313.73 ± 5.52 ** | 543.60 ± 10.69 ** | 808.20 ± 16.60 ** | 1182.00 ± 28.93 * | 1562.93 ± 37.13 * |
3.2. Changes of Weight and Relative Weight of Bursa
Item | Groups | 7 days | 14 days | 21 days | 28 days | 35 days | 42 days |
---|---|---|---|---|---|---|---|
Weight of bursae (g) | Control group | 0.19 ± 0.02 | 0.58 ± 0.05 | 1.41 ± 0.16 | 1.88 ± 0.14 | 2.84 ± 0.42 | 3.90 ± 0.46 |
Met deficiency | 019 ± 0.02 | 0.53 ± 0.05 | 1.05 ± 0.11 * | 1.12 ± 0.24 ** | 1.29 ± 0.28 ** | 2.17 ± 0.43 ** | |
Relative weight of bursae (g/kg) | Control group | 1.57 ± 0.01 | 1.69 ± 0.01 | 2.08 ± 0.01 | 1.79 ± 0.01 | 1.87 ± 0.01 | 1.93 ± 0.01 |
Met deficiency | 1.50 ± 0.02 | 1.56 ± 0.01 | 1.69 ± 0.01 ** | 1.40 ± 0.01 ** | 1.53 ± 0.01 ** | 1.38 ± 0.01 ** |
3.3. Pathological Lesions of Bursae of Fabricius
3.4. Ultrastructural Changes
3.5. Cell Cycle Phase Distribution of Bursa Cells
Groups | Item | 14 days | 28 days | 42 days |
---|---|---|---|---|
Control group | G0G1 | 82.93 ± 0.36 | 83.84 ± 0.57 | 87.95 ± 0.31 |
S | 8.55 ± 0.05 | 7.97 ± 0.57 | 5.83 ± 0.23 | |
G2M | 8.65 ± 0.24 | 8.18 ± 0.67 | 6.22 ± 0.08 | |
PI a | 17.14 ± 0.39 | 16.16 ± 0.56 | 11.93 ± 0.35 | |
Met deficiency | G0G1 | 85.91 ± 0.42 ** | 88.24 ± 0.21 ** | 93.66 ± 0.40 ** |
S | 6.96 ± 0.31 ** | 5.44 ± 0.11 ** | 2.84 ± 0.06 ** | |
G2M | 7.33 ± 0.15 ** | 6.32 ± 0.21 ** | 3.38 ± 0.44 ** | |
PI a | 14.09 ± 0.43 ** | 11.76 ± 0.21 ** | 6.22 ± 0.40 ** |
3.6. Annexin-V-FITC Staining Assay by Flow Cytometry
Group | 14 days | 28 days | 42 days |
---|---|---|---|
Control group | 1.99 ± 0.03 | 2.48 ± 0.29 | 3.19 ± 0.34 |
Met deficiency | 2.13 ± 0.10 | 3.20 ± 0.27 * | 5.74 ± 0.54 ** |
4. Discussion
5. Conclusions
Acknowledgments
Conflict of Interest
References
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Wu, B.; Cui, H.; Peng, X.; Fang, J.; Cui, W.; Liu, X. Pathology of Bursae of Fabricius in Methionine-Deficient Broiler Chickens. Nutrients 2013, 5, 877-886. https://doi.org/10.3390/nu5030877
Wu B, Cui H, Peng X, Fang J, Cui W, Liu X. Pathology of Bursae of Fabricius in Methionine-Deficient Broiler Chickens. Nutrients. 2013; 5(3):877-886. https://doi.org/10.3390/nu5030877
Chicago/Turabian StyleWu, Bangyuan, Hengmin Cui, Xi Peng, Jing Fang, Wei Cui, and Xiaodong Liu. 2013. "Pathology of Bursae of Fabricius in Methionine-Deficient Broiler Chickens" Nutrients 5, no. 3: 877-886. https://doi.org/10.3390/nu5030877
APA StyleWu, B., Cui, H., Peng, X., Fang, J., Cui, W., & Liu, X. (2013). Pathology of Bursae of Fabricius in Methionine-Deficient Broiler Chickens. Nutrients, 5(3), 877-886. https://doi.org/10.3390/nu5030877