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Alcohol Exposure Alters Mouse Lung Inflammation in Response to Inhaled Dust

Department of Environmental, Agricultural, and Occupational Health, College of Public Health, University of Nebraska Medical Center, Omaha, NE 68198, USA
VA Nebraska-Western Iowa Health Care System Research Service, Department of Veterans Affairs Medical Center, 4101 Woolworth Avenue, Omaha, NE 68105, USA
Pulmonary, Critical Care, Sleep & Allergy Division, Department of Internal Medicine, 985300 Nebraska Medical Center, Omaha, NE 68198, USA
Author to whom correspondence should be addressed.
Nutrients 2012, 4(7), 695-710;
Received: 5 March 2012 / Revised: 21 June 2012 / Accepted: 26 June 2012 / Published: 4 July 2012
(This article belongs to the Special Issue Alcohol and Health)
Alcohol exposure is associated with increased lung infections and decreased mucociliary clearance. Occupational workers exposed to dusts from concentrated animal feeding operations (CAFOs) are at risk for developing chronic inflammatory lung diseases. Agricultural worker co-exposure to alcohol and organic dust has been established, although little research has been conducted on the combination effects of alcohol and organic dusts on the lung. Previously, we have shown in a mouse model that exposure to hog dust extract (HDE) collected from a CAFO results in the activation of protein kinase C (PKC), elevated lavage fluid cytokines/chemokines including interleukin-6 (IL-6), and the development of significant lung pathology. Because alcohol blocks airway epithelial cell release of IL-6 in vitro, we hypothesized that alcohol exposure would alter mouse lung inflammatory responses to HDE. To test this hypothesis, C57BL/6 mice were fed 20% alcohol or water ad libitum for 6 weeks and treated with 12.5% HDE by intranasal inhalation method daily during the final three weeks. Bronchoalveolar lavage fluid (BALF), tracheas and lungs were collected. HDE stimulated a 2–4 fold increase in lung and tracheal PKCε (epsilon) activity in mice, but no such increase in PKCε activity was observed in dust-exposed mice fed alcohol. Similarly, alcohol-fed mice demonstrated significantly less IL-6 in lung lavage in response to dust than that observed in control mice instilled with HDE. TNFα levels were also inhibited in the alcohol and HDE-exposed mouse lung tissue as compared to the HDE only exposed group. HDE-induced lung inflammatory aggregates clearly present in the tissue from HDE only exposed animals were not visually detectable in the HDE/alcohol co-exposure group. Statistically significant weight reductions and 20% mortality were also observed in the mice co-exposed to HDE and alcohol. These data suggest that alcohol exposure depresses the ability of the lung to activate PKCε-dependent inflammatory pathways to environmental dust exposure. These data also define alcohol as an important co-exposure agent to consider in the study of inhalation injury responses. View Full-Text
Keywords: alcohol; inflammation; mortality; organic dust alcohol; inflammation; mortality; organic dust
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MDPI and ACS Style

McCaskill, M.L.; Romberger, D.J.; DeVasure, J.; Boten, J.; Sisson, J.H.; Bailey, K.L.; Poole, J.A.; Wyatt, T.A. Alcohol Exposure Alters Mouse Lung Inflammation in Response to Inhaled Dust. Nutrients 2012, 4, 695-710.

AMA Style

McCaskill ML, Romberger DJ, DeVasure J, Boten J, Sisson JH, Bailey KL, Poole JA, Wyatt TA. Alcohol Exposure Alters Mouse Lung Inflammation in Response to Inhaled Dust. Nutrients. 2012; 4(7):695-710.

Chicago/Turabian Style

McCaskill, Michael L., Debra J. Romberger, Jane DeVasure, Jessica Boten, Joseph H. Sisson, Kristina L. Bailey, Jill A. Poole, and Todd A. Wyatt. 2012. "Alcohol Exposure Alters Mouse Lung Inflammation in Response to Inhaled Dust" Nutrients 4, no. 7: 695-710.

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