Aerobic Exercise Ameliorates Myocardial Fibrosis via Affecting Vitamin D Receptor and Transforming Growth Factor-β1 Signaling in Vitamin D-Deficient Mice
Abstract
1. Introduction
2. Materials and Methods
2.1. Experimental Animals
2.2. Exercise Program
2.3. Body Weight and Body Composition
2.4. Echocardiography
2.5. Serum Biochemical Testing
2.6. Morphology
2.7. Quantitative Real-Time PCR
2.8. Western Blotting
2.9. Statistical Analysis
3. Results
3.1. The Modeling of Vitamin D-Deficient Mice
3.2. Effect of Aerobic Exercise Training on the Serum 25(OH)D Levels, Body Weight, Lean Mass and Body Fat Rate of Vitamin D-Deficient Mice
3.3. Aerobic Exercise Training Caused Myocardial Hypertrophy in Vitamin D-Deficient Mice
3.4. Aerobic Exercise Training Improved Cardiac Function in Vitamin D-Deficient Mice
3.5. Effect of Aerobic Exercise Training on the Myocardial Morphology of Vitamin D-Deficient Mice
3.6. Aerobic Exercise Training Upregulated Myocardial VDR Expression in Vitamin D-Deficient Mice
3.7. Aerobic Exercise Training Downregulated Myocardial Fibrotic Factors Expression in Vitamin D-Deficient Mice
3.8. Aerobic Exercise Training Inhibited TGF-β1-Smad2/3 Pathway in Vitamin D-Deficient Mice
3.9. Aerobic Exercise Training Downregulated Inflammatory Factors Expression in Vitamin D-Deficient Mice
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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CONS | VDDS | VDDE | |
---|---|---|---|
HW/TL (mg/mm) | 7.44 ± 0.61 | 7.65 ± 0.72 | 8.65 ± 0.85 *# |
LVW/TL (mg/mm) | 2.87 ± 0.34 | 3.00 ± 0.19 | 3.48 ± 0.38 **# |
CONS | VDDS | VDDE | |
---|---|---|---|
Ca (mmol/L) | 1.68 (1.63, 1.70) | 1.67 ± 0.04 | 1.66 (1.56, 1.69) |
P (mmol/L) | 1.97 ± 0.46 | 2.15 ± 0.33 | 2.26 ± 0.32 |
HDL-C (mmol/L) | 4.10 ± 0.47 | 3.74 ± 0.50 | 3.84 ± 0.21 |
LDL-C (mmol/L) | 1.18 ± 0.44 | 0.87 ± 0.64 | 1.32 ± 0.43 |
TG (mmol/L) | 1.12 ± 0.17 | 1.28 ± 0.17 | 1.14 ± 0.16 |
TC (mmol/L) | 2.65 ± 0.42 | 2.71 ± 0.30 | 2.81 ± 0.33 |
PTH (ng/L) | 210.52 ± 51.03 | 178.19 ± 50.39 | 225.74 ± 30.16 |
INS (ng/L) | 22.40 ± 4.27 | 22.96 ± 7.37 | 21.32 ± 4.85 |
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Cui, X.; Wang, K.; Zhang, J.; Cao, Z.-B. Aerobic Exercise Ameliorates Myocardial Fibrosis via Affecting Vitamin D Receptor and Transforming Growth Factor-β1 Signaling in Vitamin D-Deficient Mice. Nutrients 2023, 15, 741. https://doi.org/10.3390/nu15030741
Cui X, Wang K, Zhang J, Cao Z-B. Aerobic Exercise Ameliorates Myocardial Fibrosis via Affecting Vitamin D Receptor and Transforming Growth Factor-β1 Signaling in Vitamin D-Deficient Mice. Nutrients. 2023; 15(3):741. https://doi.org/10.3390/nu15030741
Chicago/Turabian StyleCui, Xiaoning, Ke Wang, Jinghua Zhang, and Zhen-Bo Cao. 2023. "Aerobic Exercise Ameliorates Myocardial Fibrosis via Affecting Vitamin D Receptor and Transforming Growth Factor-β1 Signaling in Vitamin D-Deficient Mice" Nutrients 15, no. 3: 741. https://doi.org/10.3390/nu15030741
APA StyleCui, X., Wang, K., Zhang, J., & Cao, Z.-B. (2023). Aerobic Exercise Ameliorates Myocardial Fibrosis via Affecting Vitamin D Receptor and Transforming Growth Factor-β1 Signaling in Vitamin D-Deficient Mice. Nutrients, 15(3), 741. https://doi.org/10.3390/nu15030741