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Article

Dietary Alaska Pollack Protein Induces Acute and Sustainable Skeletal Muscle Hypertrophy in Rats

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The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan
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Food Function R&D Center, Nippon Suisan Kaisha Ltd., Tokyo 105-8676, Japan
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Laboratory of Nutrition Science, Division of Applied Bioscience, Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan
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Department of Applied Biological Chemistry, School of Agriculture, Utsunomiya University, 350 Minemachi, Utsunomiya 321-8505, Japan
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Physiology of Domestic Animals, Faculty of Agriculture and Life Science, Hirosaki University, 3 Bunkyo-cho, Hirosaki 036-8561, Japan
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Laboratory of Food Chemistry, Yokohama University of Pharmacy, Yokohama 245-0066, Japan
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Food Functionality Laboratory, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
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Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa 252-0880, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Philip J. Atherton
Nutrients 2022, 14(3), 547; https://doi.org/10.3390/nu14030547
Received: 10 December 2021 / Revised: 7 January 2022 / Accepted: 14 January 2022 / Published: 27 January 2022
(This article belongs to the Section Proteins and Amino Acids)
Our previous studies suggested that Alaska pollack protein (APP) intake increases skeletal muscle mass and that it may cause a slow-to-fast shift in muscle fiber type in rats fed a high-fat diet after 56 days of feeding. In this study, we explored whether dietary APP induces acute and sustainable skeletal muscle hypertrophy in rats fed a normal-fat diet. Male 5-week-old Sprague–Dawley rats were divided into four groups and fed a purified ingredient-based high-fat diet or a purified ingredient-based normal-fat diet with casein or APP, containing the same amount of crude protein. Dietary APP significantly increased gastrocnemius muscle mass (105~110%) after 2, 7 days of feeding, regardless of dietary fat content. Rats were separated into two groups and fed a normal-fat diet with casein or APP. Dietary APP significantly increased gastrocnemius muscle mass (110%) after 56 days of feeding. Dietary APP significantly increased the cross-sectional area of the gastrocnemius skeletal muscle and collagen-rich connective tissue after 7 days of feeding. It decreased the gene expression of Mstn /Myostatin, Trim63/MuRF1, and Fbxo32/atrogin-1, but not other gene expression, such as serum IGF-1 after 7 days of feeding. No differences were observed between casein and APP groups with respect to the percentage of Type I, Type IIA, and Type IIX or IIB fibers, as determined by myosin ATPase staining after 7 days of feeding. In the similar experiment, the puromycin-labeled peptides were not different between dietary casein and APP after 2 days of feeding. These results demonstrate that APP induces acute and sustainable skeletal muscle hypertrophy in rats, regardless of dietary fat content. Dietary APP, as a daily protein source, may be an approach for maintaining or increasing muscle mass. View Full-Text
Keywords: fish protein; skeletal muscle; muscle fiber type; protein source; rat fish protein; skeletal muscle; muscle fiber type; protein source; rat
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MDPI and ACS Style

Uchida, K.; Fujitani, M.; Mizushige, T.; Kawabata, F.; Hayamizu, K.; Uozumi, K.; Hara, Y.; Sawai, M.; Uehigashi, R.; Okada, S.; Goto-Inoue, N.; Morisasa, M.; Kishida, T. Dietary Alaska Pollack Protein Induces Acute and Sustainable Skeletal Muscle Hypertrophy in Rats. Nutrients 2022, 14, 547. https://doi.org/10.3390/nu14030547

AMA Style

Uchida K, Fujitani M, Mizushige T, Kawabata F, Hayamizu K, Uozumi K, Hara Y, Sawai M, Uehigashi R, Okada S, Goto-Inoue N, Morisasa M, Kishida T. Dietary Alaska Pollack Protein Induces Acute and Sustainable Skeletal Muscle Hypertrophy in Rats. Nutrients. 2022; 14(3):547. https://doi.org/10.3390/nu14030547

Chicago/Turabian Style

Uchida, Kenji, Mina Fujitani, Takafumi Mizushige, Fuminori Kawabata, Kohsuke Hayamizu, Keisuke Uozumi, Yuma Hara, Mariko Sawai, Ryota Uehigashi, Shinji Okada, Naoko Goto-Inoue, Mizuki Morisasa, and Taro Kishida. 2022. "Dietary Alaska Pollack Protein Induces Acute and Sustainable Skeletal Muscle Hypertrophy in Rats" Nutrients 14, no. 3: 547. https://doi.org/10.3390/nu14030547

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