Aruncus dioicus var. kamtschaticus Extract Ameliorates Psoriasis-like Skin Inflammation via Akt/mTOR and JAK2/STAT3 Signaling Pathways in a Murine Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material Preparation and Extraction
2.2. Animal Study
2.3. Cell Culture and Treatment
2.4. Cell Viability Assay
2.5. Immunohistochemistry
2.6. Quantitative Reverse Transcription-Polymerase Chain Reaction (RT–qPCR)
2.7. Western Blot Analysis
2.8. Statistical Analysis
3. Results
3.1. Topical Application of ADE Reduced the Development of Psoriatic Phenotypes and Improved Skin Conditions
3.2. ADE Treatment Attenuates the Infiltration of Immune Cells into Psoriatic Lesions by Suppressing the Secretion of Psoriatic Markers in Mouse Skin
3.3. ADE Treatment Inhibits the Expression of Pro-Inflammatory Cytokines in HaCaT Cells
3.4. ADE Treatment Modulates JAK2/STAT3 and Akt/mTOR Signaling Pathways
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Target Gene | Primer Sequence (5′-3′) | |
---|---|---|
Forward | Reverse | |
hIL-1β | 5′-TGAGCTCGCCAGTGAAATGA-3′ | 5′-AGATTCGTAGCTGGATGCCG-3′ |
hIL-23A | 5′ACAGAAGCTCTGCACACTGG-3′ | 5′-GTTGTCCCTGAGTCCTTGGG-3′ |
hCXCL1 | 5′-ATTCACCCCAAGAACATCCA-3′ | 5′-TGGATTTGTCACTGTTCAGCA-3′ |
hCXCL2 | 5′-GCAGGGAATTCACCTCAAGA-3′ | 5′-TGGATTTGCCATTTTTCAGC-3′ |
hCXCL10 | 5′-TGCCATTCTGATTTGCTGCC-3′ | 5′-ATGCAGGTACAGCGTACAGTT-3′ |
hCCL20 | 5′-TGTCAGTGCTGCTACTCCAC-3′ | 5′-CCGTGTGAAGCCCACAATAA-3′ |
hS100A8 | 5′-AAGGGGAATTTCCATGCCGT-3″ | 5′-AGGACACTCGGTCTCTAGCA-3′ |
hS100A9 | 5′-CATGCTGATGGCGAGGCTAA-3′ | 5′-GCCTCGTGCATCTTCTCGTG-3′ |
hGAPDH | 5′-GAAGGTGAAGGTCGGAGTC-3′ | 5′-GAAGATGGTGATGGGATTTC-3′ |
mIL-1β | 5′-CAGGCAGGCAGTATCACTCA-3′ | 5′-AGGCCACAGGTATTTTGTCG-3′ |
mIL-23A | 5′-CCATGGAGCAACTTCACACC-3′ | 5′-CTGGAGGCTTCGAAGGATCT-3′ |
mTNF-α | 5′-TCTTCTCGAACCCCGAGTGA-3′ | 5′-CCTCTGATGGCACCACCAG-3′ |
mCXCL1 | 5′-GTCAGTGCCTGCAGACCAT-3′ | 5′-AACCAAGGGAGCTTCAGGG-3′ |
mCXCL2 | 5′-ACATCCAGAGCTTGAGTGTG-3′ | 5′-GCCTTGCCTTTGTTCAGTATCT-3′ |
mCXCL10 | 5′-TGAATCCGGAATCTAAGACCATCAA-3′ | 5′-AGGACTAGCCATCCACTGGGTAAAG-3′ |
mCCL20 | 5′-CGACTGTTGCCTCTCGTACA-3′ | 5′-AGCCCTTTTCACCCAGTTCT-3′ |
mS100A8 | 5′-ATGCCGTCTGAACTGGAGAA-3′ | 5′-TAGAGGGCATGGTGATTTCC-3′ |
mS100A9 | 5′-CAGCATAACCACCATCATCG-3′ | 5′-AAGGTTGCCAACTGTGCTTC-3′ |
mGAPDH | 5′-CATGGCCTTCCGTGTTCCTA-3′ | 5′-ACTTGGCAGGTTTCTCCAGG-3′ |
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Dorjsembe, B.; Joo, H.; Nho, C.; Ham, J.; Kim, J.-C. Aruncus dioicus var. kamtschaticus Extract Ameliorates Psoriasis-like Skin Inflammation via Akt/mTOR and JAK2/STAT3 Signaling Pathways in a Murine Model. Nutrients 2022, 14, 5094. https://doi.org/10.3390/nu14235094
Dorjsembe B, Joo H, Nho C, Ham J, Kim J-C. Aruncus dioicus var. kamtschaticus Extract Ameliorates Psoriasis-like Skin Inflammation via Akt/mTOR and JAK2/STAT3 Signaling Pathways in a Murine Model. Nutrients. 2022; 14(23):5094. https://doi.org/10.3390/nu14235094
Chicago/Turabian StyleDorjsembe, Banzragch, Haneul Joo, Chuwon Nho, Jungyeob Ham, and Jin-Chul Kim. 2022. "Aruncus dioicus var. kamtschaticus Extract Ameliorates Psoriasis-like Skin Inflammation via Akt/mTOR and JAK2/STAT3 Signaling Pathways in a Murine Model" Nutrients 14, no. 23: 5094. https://doi.org/10.3390/nu14235094
APA StyleDorjsembe, B., Joo, H., Nho, C., Ham, J., & Kim, J.-C. (2022). Aruncus dioicus var. kamtschaticus Extract Ameliorates Psoriasis-like Skin Inflammation via Akt/mTOR and JAK2/STAT3 Signaling Pathways in a Murine Model. Nutrients, 14(23), 5094. https://doi.org/10.3390/nu14235094