A Hydrodistillate of Gynostemma pentaphyllum and Damulin B Prevent Cisplatin-Induced Nephrotoxicity In Vitro and In Vivo via Regulation of AMPKα1 Transcription
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Antibodies
2.2. Preparation of Cell Culture Media Containing a Hydrodistillate of G. pentaphyllum Leaves
2.3. Animal Experiments
2.4. Assessment of Blood Parameters and Histopathological Analysis
2.5. Primers Used for Reverse Transcriptase-PCR
2.6. Miscellaneous Experiments
3. Results
3.1. GPHD Protects HEK293 Cells from Cisplatin-INDUCED Cytotoxicity
3.2. Cisplatin Decreases the Expression of AMPKα1 at the Transcriptional Level
3.3. GPHD Protects HEK293 Cells from Cisplatin-Induced Cytotoxicity through AMPKα1
3.4. GPHD Suppresses Cisplatin-Generated Oxidative Stress via AMPKα1
3.5. Intake of GPHD Prevents Cisplatin-Induced AKI in Mice
3.6. Damulin B Prevents Cisplatin-Induced Cytotoxicity in HEK293 Cells and Cisplatin-Induced AKI in Mice
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Conflicts of Interest
References
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Song, M.; Kim, M.; Hoang, D.H.; Kovale, L.M.; Lee, J.; Kim, Y.; Lee, C.; Hong, J.; Park, S.; Choe, W.; et al. A Hydrodistillate of Gynostemma pentaphyllum and Damulin B Prevent Cisplatin-Induced Nephrotoxicity In Vitro and In Vivo via Regulation of AMPKα1 Transcription. Nutrients 2022, 14, 4997. https://doi.org/10.3390/nu14234997
Song M, Kim M, Hoang DH, Kovale LM, Lee J, Kim Y, Lee C, Hong J, Park S, Choe W, et al. A Hydrodistillate of Gynostemma pentaphyllum and Damulin B Prevent Cisplatin-Induced Nephrotoxicity In Vitro and In Vivo via Regulation of AMPKα1 Transcription. Nutrients. 2022; 14(23):4997. https://doi.org/10.3390/nu14234997
Chicago/Turabian StyleSong, Minhyeok, Minseok Kim, Dang Hieu Hoang, Lochana Mangesh Kovale, Jihyun Lee, Youngjoo Kim, Changhyun Lee, Jongki Hong, Sungchul Park, Wonchae Choe, and et al. 2022. "A Hydrodistillate of Gynostemma pentaphyllum and Damulin B Prevent Cisplatin-Induced Nephrotoxicity In Vitro and In Vivo via Regulation of AMPKα1 Transcription" Nutrients 14, no. 23: 4997. https://doi.org/10.3390/nu14234997