Moderate Hyperglycemia-Preventive Effect and Mechanism of Action of Periplaneta americana Oligosaccharides in Streptozotocin-Induced Diabetic Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Chemicals
2.2. Preparation of PAOS
2.3. Animals
2.4. Establishment of the DM Model and PAOS Treatment
2.5. Fasting Serum Insulin Levels
2.6. Measurement of Cytokines, Serum Parameters and Organ Indexes
2.7. Measurement of Liver Function and Antioxidant Parameters
2.8. Immunohistochemistry Staining
2.9. Western Blot
2.10. Microbial Community Analysis
2.11. Statistical Analysis
3. Results
3.1. Effects of PAOS on DM Were Revealed by Body Weight, FBG, Insulin and HOMA-β
3.2. PAOS Regulated Immune Responses in DM Mice
3.3. PAOS Reduced Serum Parameterslevels
3.4. PAOS Benefited Liver Function and Antioxidant Parameters in DM Mice
3.5. PAOS Improved Islet β Cell Function and Downregulated Its Apoptosis in DM Mice
3.6. PAOS Regulated Signal Pathways in the Livers of TIDM Mice
3.7. PAOS Regulated the Gut Microbiota in Control Mice
3.8. PAOS Ameliorated on Gut Microbiota in DM Mice
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Lu, K.; He, Y.; Wu, C.; Bao, J. Moderate Hyperglycemia-Preventive Effect and Mechanism of Action of Periplaneta americana Oligosaccharides in Streptozotocin-Induced Diabetic Mice. Nutrients 2022, 14, 4620. https://doi.org/10.3390/nu14214620
Lu K, He Y, Wu C, Bao J. Moderate Hyperglycemia-Preventive Effect and Mechanism of Action of Periplaneta americana Oligosaccharides in Streptozotocin-Induced Diabetic Mice. Nutrients. 2022; 14(21):4620. https://doi.org/10.3390/nu14214620
Chicago/Turabian StyleLu, Kaimin, Yufei He, Chuanfang Wu, and Jinku Bao. 2022. "Moderate Hyperglycemia-Preventive Effect and Mechanism of Action of Periplaneta americana Oligosaccharides in Streptozotocin-Induced Diabetic Mice" Nutrients 14, no. 21: 4620. https://doi.org/10.3390/nu14214620