Anti-Obesity Effects of Aqueous Extracts of Sunbanghwalmyung-Eum in High-Fat- and High-Cholesterol-Diet-Induced Obese C57BL/6J Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Preparation of SBH Extract and HPLC Analysis of SBH
2.3. Animal Experiment
2.4. Plasma Biochemical Parameter Analysis
2.5. OGTT
2.6. H&E Staining
2.7. Western Blot Analysis
2.8. Statistical Analysis
3. Results
3.1. Quantification of the Eight Marker Compounds in SBH
3.2. SBH Reduced HFHCD-Induced Body Weight Gain
3.3. SBH Reduced HFHCD-Induced Liver Disorder Factor and Lipid Parameters
3.4. SBH Suppressed HFHCD-Induced Glucose Tolerance and Plasma Biomarkers of Diabetes
3.5. SBH Reversed HFHCD-Induced Regulation of Signaling Molecules
3.6. SBH Reversed HFHCD-Induced Inhibition of AMPK and HSL Phosphorylation
3.7. SBH Reduced HFHCD-Induced Expression of Inflammatory Cytokines
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primary Antibody | Dilution | Catalogue No. | Company | Secondary Antibody | Dilution | Catalogue No. | Company |
---|---|---|---|---|---|---|---|
AMPKα | 1:1000 | sc-5298 | Santa | Goat-anti-rabbit | 1:5000 | ADI-SAB-300 | Enzo |
C/EBPα | 1:1000 | sc-365318 | Santa | Goat-anti-mouse | 1:5000 | ADI-SAB-100 | Enzo |
HSL | 1:500 | ab45422 | Abcam | Goat-anti-rabbit | 1:5000 | ADI-SAB-300 | Enzo |
IL-1β | 1:1000 | 12242s | Cell Signaling | Goat-anti-mouse | 1:5000 | ADI-SAB-100 | Enzo |
MCP1 | 1:1000 | ab25124 | Abcam | Goat-anti-rabbit | 1:5000 | ADI-SAB-300 | Enzo |
PPARγ | 1:500 | 2430s | Cell Signaling | Goat-anti-rabbit | 1:5000 | ADI-SAB-300 | Enzo |
pAMPKα | 1:500 | 2535s | Cell Signaling | Goat-anti-rabbit | 1:5000 | ADI-SAB-300 | Enzo |
pHSL | 1:500 | 4139s | Cell Signaling | Goat-anti-rabbit | 1:5000 | ADI-SAB-300 | Enzo |
TNFα | 1:1000 | sc-52746 | Santa | Goat-anti-mouse | 1:5000 | ADI-SAB-100 | Enzo |
GAPDH | 1:1000 | Sc-32233 | Santa | Goat-anti-mouse | 1:5000 | ADI-SAB-100 | Enzo |
Tissue Type | Nor | HFHC | Sim | SBH |
---|---|---|---|---|
Liver | 1.288 ± 0.060 | 2.621 ± 0.284 ### | 1.633 ± 0.079 *** | 1.945 ± 0.083 * |
Adipose tissue | 0.618 ± 0.037 | 2.601 ± 0.158 ### | 1.483 ± 0.061 ** | 1.694 ± 0.115 0.057 |
Kidney | 0.158 ± 0.026 | 0.181 ± 0.010 | 0.167 ± 0.021 | 0.182 ± 0.013 |
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Kim, H.-L.; Ahn, Y.M.; Lee, S.M.; Seo, C.-S.; Park, S.-H.; Bang, O.-S.; Jung, J. Anti-Obesity Effects of Aqueous Extracts of Sunbanghwalmyung-Eum in High-Fat- and High-Cholesterol-Diet-Induced Obese C57BL/6J Mice. Nutrients 2022, 14, 2929. https://doi.org/10.3390/nu14142929
Kim H-L, Ahn YM, Lee SM, Seo C-S, Park S-H, Bang O-S, Jung J. Anti-Obesity Effects of Aqueous Extracts of Sunbanghwalmyung-Eum in High-Fat- and High-Cholesterol-Diet-Induced Obese C57BL/6J Mice. Nutrients. 2022; 14(14):2929. https://doi.org/10.3390/nu14142929
Chicago/Turabian StyleKim, Hye-Lin, You Mee Ahn, So Min Lee, Chang-Seob Seo, Seong-Hwan Park, Ok-Sun Bang, and Jeeyoun Jung. 2022. "Anti-Obesity Effects of Aqueous Extracts of Sunbanghwalmyung-Eum in High-Fat- and High-Cholesterol-Diet-Induced Obese C57BL/6J Mice" Nutrients 14, no. 14: 2929. https://doi.org/10.3390/nu14142929