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High Fat Activates O-GlcNAcylation and Affects AMPK/ACC Pathway to Regulate Lipid Metabolism

1
The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs), The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao 266003, China
2
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao 266237, China
*
Author to whom correspondence should be addressed.
Academic Editors: Paul Holvoet and Susanna Iossa
Nutrients 2021, 13(6), 1740; https://doi.org/10.3390/nu13061740
Received: 29 March 2021 / Revised: 5 May 2021 / Accepted: 13 May 2021 / Published: 21 May 2021
(This article belongs to the Section Nutrition and Metabolism)
A high-fat diet often leads to excessive fat deposition and adversely affects the organism. However, the mechanism of liver fat deposition induced by high fat is still unclear. Therefore, this study aimed at acetyl-CoA carboxylase (ACC) to explore the mechanism of excessive liver deposition induced by high fat. In the present study, the ORF of ACC1 and ACC2 were cloned and characterized. Meanwhile, the mRNA and protein of ACC1 and ACC2 were increased in liver fed with a high-fat diet (HFD) or in hepatocytes incubated with oleic acid (OA). The phosphorylation of ACC was also decreased in hepatocytes incubated with OA. Moreover, AICAR dramatically improved the phosphorylation of ACC, and OA significantly inhibited the phosphorylation of the AMPK/ACC pathway. Further experiments showed that OA increased global O-GlcNAcylation and agonist of O-GlcNAcylation significantly inhibited the phosphorylation of AMPK and ACC. Importantly, the disorder of lipid metabolism caused by HFD or OA could be rescued by treating CP-640186, the dual inhibitor of ACC1 and ACC2. These observations suggested that high fat may activate O-GlcNAcylation and affect the AMPK/ACC pathway to regulate lipid synthesis, and also emphasized the importance of the role of ACC in lipid homeostasis. View Full-Text
Keywords: large yellow croaker; acetyl-CoA carboxylase; O-GlcNAc; AMPK; lipid metabolism large yellow croaker; acetyl-CoA carboxylase; O-GlcNAc; AMPK; lipid metabolism
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MDPI and ACS Style

Pang, Y.; Xu, X.; Xiang, X.; Li, Y.; Zhao, Z.; Li, J.; Gao, S.; Liu, Q.; Mai, K.; Ai, Q. High Fat Activates O-GlcNAcylation and Affects AMPK/ACC Pathway to Regulate Lipid Metabolism. Nutrients 2021, 13, 1740. https://doi.org/10.3390/nu13061740

AMA Style

Pang Y, Xu X, Xiang X, Li Y, Zhao Z, Li J, Gao S, Liu Q, Mai K, Ai Q. High Fat Activates O-GlcNAcylation and Affects AMPK/ACC Pathway to Regulate Lipid Metabolism. Nutrients. 2021; 13(6):1740. https://doi.org/10.3390/nu13061740

Chicago/Turabian Style

Pang, Yuning, Xiang Xu, Xiaojun Xiang, Yongnan Li, Zengqi Zhao, Jiamin Li, Shengnan Gao, Qiangde Liu, Kangsen Mai, and Qinghui Ai. 2021. "High Fat Activates O-GlcNAcylation and Affects AMPK/ACC Pathway to Regulate Lipid Metabolism" Nutrients 13, no. 6: 1740. https://doi.org/10.3390/nu13061740

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