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Article

Association of Gut Microbiome and Vitamin D Deficiency in Knee Osteoarthritis Patients: A Pilot Study

1
Department of Orthopaedics, Christian Medical College, Vellore, Tamil Nadu 632004, India
2
Microbiology and Mycology Program, Biomedical Sciences Institute (ICBM), School of Medicine, University of Chile, Santiago Región Metropolitana 8380418, Chile
3
Department of Orthopaedics, All India Institute of Medical Sciences, Bhubaneswar 110608, India
4
Center of Excellence for Clinical Microbiome Research, All India Institute of Medical Sciences, Bhubaneswar 110608, India
5
Department of Biochemistry, All India Institute of Medical Sciences, Bhubaneswar 110608, India
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Current affiliation: Department of Orthopaedics, Royal Darwin Hospital, 105 Rocklands Drive, Tiwi NT–0810, Australia.
Academic Editor: Patrizia D’Amelio
Nutrients 2021, 13(4), 1272; https://doi.org/10.3390/nu13041272
Received: 21 January 2021 / Revised: 3 March 2021 / Accepted: 4 March 2021 / Published: 13 April 2021
(This article belongs to the Special Issue Calcium, Vitamin D and Aging)
Background: Few preclinical studies have shown that Knee osteoarthritis (KOA) is linked to gut microbiome dysbiosis and chronic inflammation. This pilot study was designed to look at the gut microbiome composition in KOA patients and normal individuals with or without vitamin D deficiency (VDD, serum vitamin D <30 ng/mL). Methods: This pilot study was conducted prospectively in 24 participants. The faecal samples of all the participants were taken for DNA extraction. The V3-V4 region of 16s rRNA was amplified, and the library was prepared and sequenced on the Illumina Miseq platform. Results: The mean (±SD) age was 45.5 (±10.2) years with no defined comorbidities. Of 447 total Operational Taxonomic Units (OTUs), a differential abundance of 16 nominally significant OTUs between the groups was observed. Linear discriminate analysis (LEfSe) revealed a significant difference in bacteria among the study groups. Pseudobutyrivibrio and Odoribacter were specific for VDD, while Parabacteroides, Butyricimonas and Gordonibacter were abundant in the KOA_VDD group, and Peptococcus, Intestimonas, Delftia and Oribacterium were abundant in the KOA group. About 80% of bacterial species were common among different groups and hence labelled as core bacterial species. However, the core microbiome of KOA and VDD groups were not seen in the KOA_VDD group, suggesting that these bacterial groups were affected by the interaction of the KOA and VDD factors. Conclusion: Parabacteroides, Butyricimonas, Pseudobutyrivibrio, Odoribacter and Gordonibacter are the predominant bacteria in vitamin D deficient patients with or without KOA. Together these results indicate an association between the gut microbiome, vitamin D and knee osteoarthritis. View Full-Text
Keywords: knee osteoarthritis; vitamin D; gut microbiome knee osteoarthritis; vitamin D; gut microbiome
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MDPI and ACS Style

Ramasamy, B.; Magne, F.; Tripathy, S.K.; Venugopal, G.; Mukherjee, D.; Balamurugan, R. Association of Gut Microbiome and Vitamin D Deficiency in Knee Osteoarthritis Patients: A Pilot Study. Nutrients 2021, 13, 1272. https://doi.org/10.3390/nu13041272

AMA Style

Ramasamy B, Magne F, Tripathy SK, Venugopal G, Mukherjee D, Balamurugan R. Association of Gut Microbiome and Vitamin D Deficiency in Knee Osteoarthritis Patients: A Pilot Study. Nutrients. 2021; 13(4):1272. https://doi.org/10.3390/nu13041272

Chicago/Turabian Style

Ramasamy, Boopalan, Fabien Magne, Sujit K. Tripathy, Giriprasad Venugopal, Diptasree Mukherjee, and Ramadass Balamurugan. 2021. "Association of Gut Microbiome and Vitamin D Deficiency in Knee Osteoarthritis Patients: A Pilot Study" Nutrients 13, no. 4: 1272. https://doi.org/10.3390/nu13041272

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