Perilla frutescens Extracts Enhance DNA Repair Response in UVB Damaged HaCaT Cells
Department of Food and Nutrition, Hannam University, Daejeon 34054, Korea
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Academic Editors: Jean Christopher Chamcheu, Anthony L. Walker and Felicite Noubissi-Kamdem
Nutrients 2021, 13(4), 1263; https://doi.org/10.3390/nu13041263
Received: 19 March 2021
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Revised: 7 April 2021
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Accepted: 9 April 2021
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Published: 12 April 2021
(This article belongs to the Special Issue Natural and Synthetic Bioactives for Skin Health, Disease and Management)
Physiological processes in skin are associated with exposure to UV light and are essential for skin maintenance and regeneration. Here, we investigated whether the leaf and callus extracts of Perilla frutescens (Perilla), a well-known Asian herb, affect DNA damage response and repair in skin and keratinocytes exposed to Untraviolet B (UVB) light. First, we examined the protective effects of Perilla leaf extracts in UVB damaged mouse skin in vivo. Second, we cultured calluses using plant tissue culture technology, from Perilla leaf explant and then examined the effects of the leaf and callus extracts of Perilla on UVB exposed keratinocytes. HaCaT cells treated with leaf and callus Perilla extracts exhibited antioxidant activities, smaller DNA fragment tails, and enhanced colony formation after UVB exposure. Interestingly, keratinocytes treated with the leaf and callus extracts of Perilla showed G1/S cell cycle arrest, reduced protein levels of cyclin D1, Cyclin Dependent Kinase 6 (CDK6), and γH2AX, and enhanced levels of phosphorylated checkpoint kinase 1 (pCHK1) following UVB exposure. These observations suggest that the leaf and callus extracts of Perilla are candidate nutraceuticals for the prevention of keratinocyte aging.
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MDPI and ACS Style
Lee, H.; Park, E. Perilla frutescens Extracts Enhance DNA Repair Response in UVB Damaged HaCaT Cells. Nutrients 2021, 13, 1263. https://doi.org/10.3390/nu13041263
AMA Style
Lee H, Park E. Perilla frutescens Extracts Enhance DNA Repair Response in UVB Damaged HaCaT Cells. Nutrients. 2021; 13(4):1263. https://doi.org/10.3390/nu13041263
Chicago/Turabian StyleLee, Hyuna, and Eunmi Park. 2021. "Perilla frutescens Extracts Enhance DNA Repair Response in UVB Damaged HaCaT Cells" Nutrients 13, no. 4: 1263. https://doi.org/10.3390/nu13041263
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