Polydatin Prevents Calcium Pyrophosphate Crystal-Induced Arthritis in Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice
2.2. CPP Crystal-Induced Arthritis Development
2.3. Drugs
2.4. Treatment with PD and Colchicine
2.5. A Priori Sample Size Calculation, Primary and Secondary Outcomes
2.6. Histological Assessment
2.7. Ultrasound Assessment
2.8. RNA Extraction from Ankle Joint and RT-PCR
- IL-1ß Forward: 5′-CGCAGCAGCACATCAACAAG-3′
- Reverse: 5′-GTGCTCATGTCCTCATCCTG-3′
- CXCL1 Forward: 5′-ATCCAGAGCTTGAAGGTGTTG-3′
- Reverse: 5′-GTCTGTCTTCTTTCTCCGTTACTT-3′
- 18. S Forward: 5′ GGGAGCCTGAGAAACGGC 3′
- Reverse: 5′ GGGTCGGGAGTGGGTAATTT 3′
2.9. Serum Cytokine Determination
2.10. Statistical Analyses
3. Results
3.1. Prophylactic Oral Treatment with PD Prevents CPP Crystal-Induced Arthritis in Mice
3.2. Therapeutic Oral Treatment with PD Does Not Affect CPP Crystal-Induced Arthritis in Mice
3.3. Ultrasound Evaluation of CPP Crystal-Induced Arthritis in Mice Treated Prophylactically with Polydatin
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
- Oliviero, F.; Bindoli, S.; Scanu, A.; Feist, E.; Doria, A.; Galozzi, P.; Sfriso, P. Autoinflammatory Mechanisms in Crystal-Induced Arthritis. Front. Med. 2020, 7, 166. [Google Scholar] [CrossRef]
- Abhishek, A.; Neogi, T.; Choi, H.; Doherty, M.; Rosenthal, A.K.; Terkeltaub, R. Unmet needs and the path forward in joint disease associated with calcium pyrophosphate crystal deposition. Arthritis Rheumatol. 2018, 70, 1182–1191. [Google Scholar] [CrossRef][Green Version]
- Andrés, M.; Sivera, F.; Pascual, E. Therapy for CPPD: Options and evidence. Curr. Rheumatol. Rep. 2018, 20, 31. [Google Scholar] [CrossRef]
- Abhishek, A.; Doherty, M. Update on calcium pyrophosphate deposition. Clin. Exp. Rheumatol. 2016, 34, 32–38. [Google Scholar]
- Zhang, W.; Doherty, M.; Pascual, E.; Barskova, V.; Guerne, P.A.; Jansen, T.L.; Leeb, B.F.; Perez-Ruiz, F.; Pimentao, J.; Punzi, L.; et al. EULAR recommendations for calcium pyrophosphate deposition. Part II: Management. Ann. Rheum. Dis. 2011, 70, 571–575. [Google Scholar] [CrossRef]
- Tang, J.; Li, Y.; Wang, J.; Wu, Q.; Yan, H. Polydatin suppresses the development of lung inflammation and fibrosis by inhibiting activation of the NACHT domain-, leucine-rich repeat-, and pyd-containing protein 3 inflammasome and the nuclear factor-κB pathway after Mycoplasma pneumoniae infection. J. Cell Biochem. 2019, 120, 10137–10144. [Google Scholar] [CrossRef] [PubMed]
- Lv, T.; Shen, L.; Yang, L.; Diao, W.; Yang, Z.; Zhang, Y.; Yu, S.; Li, Y. Polydatin ameliorates dextran sulfate sodium-induced colitis by decreasing oxidative stress and apoptosis partially via Sonic hedgehog signaling pathway. Int. Immunopharmacol. 2018, 64, 256–263. [Google Scholar] [CrossRef]
- Martano, M.; Stiuso, P.; Facchiano, A.; De Maria, S.; Vanacore, D.; Restucci, B.; Rubini, C.; Caraglia, M.; Ravagnan, G.; Lo Muzio, L. Aryl hydrocarbon receptor, a tumor grade-associated marker of oral cancer, is directly downregulated by polydatin: A pilot study. Oncol. Rep. 2018, 40, 1435–1442. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Ravagnan, G.; De Filippis, A.; Cartenì, M.; De Maria, S.; Cozza, V.; Petrazzuolo, M.; Tufano, M.A.; Donnarumma, G. Polydatin, a natural precursor of resveratrol, induces β-defensin production and reduces inflammatory response. Inflammation 2013, 36, 26–34. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Oliviero, F.; Zamudio-Cuevas, Y.; Belluzzi, E.; Andretto, L.; Scanu, A.; Favero, M.; Ramonda, R.; Ravagnan, G.; López-Reyes, A.; Spinella, P.; et al. Polydatin and resveratrol inhibit the inflammatory process induced by urate and pyrophosphate crystals in thp-1 cells. Foods 2019, 8, 560. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Reber, L.L.; Starkl, P.; Balbino, B.; Sibilano, R.; Gaudenzio, N.; Rogalla, S.; Sensarn, S.; Kang, D.; Raghu, H.; Sokolove, J.; et al. The tyrosine kinase inhibitor imatinib mesylate suppresses uric acid crystal-induced acute gouty arthritis in mice. PLoS ONE 2017, 12, e0185704. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Livak, K.J.; Schmittgen, T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)). Method Methods 2001, 25, 402–408. [Google Scholar] [CrossRef]
- Galozzi, P.; Maschio, L.; Carraro, S.; Scanu, A.; Facco, M.; Oliviero, F. M2 macrophages as resolvers of crystal-induced inflammation. Rheumatology 2021. [Google Scholar] [CrossRef]
- Şöhretoğlu, D.; Baran, M.Y.; Arroo, R.; Kuruüzüm-Uz, A. Recent advances in chemistry, therapeutic properties and sources of polydatin. Phytochem. Rev. 2018, 17, 973–1005. [Google Scholar] [CrossRef]
- Romero-Pérez, A.I.; Ibern-Gómez, M.; Lamuela-Raventós, R.M.; de La Torre-Boronat, M.C. Piceid, the major resveratrol derivative in grape juices. J. Agric. Food Chem. 1999, 47, 1533–1536. [Google Scholar] [CrossRef]
- Liu, Y.L.; Chen, B.Y.; Nie, J.; Zhao, G.H.; Zhuo, J.Y.; Yuan, J.; Li, Y.C.; Wang, L.L.; Chen, Z.W. Polydatin prevents bleomycin-induced pulmonary fibrosis by inhibiting the TGF-β/Smad/ERK signaling pathway. Exp. Med. 2020, 20, 62. [Google Scholar] [CrossRef] [PubMed]
- Gu, L.; Liu, J.; Xu, D.; Lu, Y. Polydatin prevents LPS-induced acute kidney injury through inhibiting inflammatory and oxidative responses. Microb. Pathog. 2019, 137, 103688. [Google Scholar] [CrossRef] [PubMed]
- Liu, T.; Zhang, L.; Joo, D.; Sun, S.C. NF-κB signaling in inflammation. Signal Transduct Target Ther. 2017, 2, 17023. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Park, B.; Jo, K.; Lee, T.G.; Hyun, S.W.; Kim, J.S.; Kim, C.S. Polydatin Inhibits NLRP3 Inflammasome in Dry Eye Disease by Attenuating Oxidative Stress and Inhibiting the NF-κB Pathway. Nutrients 2019, 11, 2792. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Tang, S.; Tang, Q.; Jin, J.; Zheng, G.; Xu, J.; Huang, W.; Li, X.; Shang, P.; Liu, H. Polydatin inhibits the IL-1β-induced inflammatory response in human osteoarthritic chondrocytes by activating the Nrf2 signaling pathway and ameliorates murine osteoarthritis. Food Funct. 2018, 9, 1701–1712. [Google Scholar] [CrossRef]
- Tan, Y.Y.; Chen, L.X.; Fang, L.; Zhang, Q. Cardioprotective effects of polydatin against myocardial injury in diabetic rats via inhibition of NADPH oxidase and NF-κB activities. BMC Complement Med. 2020, 20, 378. [Google Scholar] [CrossRef]
- Zhao, X.J.; Yu, H.W.; Yang, Y.Z.; Wu, W.Y.; Chen, T.Y.; Jia, K.K.; Kang, L.L.; Jiao, R.Q.; Kong, L.D. Polydatin prevents fructose-induced liver inflammation and lipid deposition through increasing miR-200a to regulate Keap1/Nrf2 pathway. Redox Biol. 2018, 18, 124–137. [Google Scholar] [CrossRef] [PubMed]
- Li, R.; Li, J.; Huang, Y.; Li, H.; Yan, S.; Lin, J.; Chen, Y.; Wu, L.; Liu, B.; Wang, G.; et al. Polydatin attenuates diet-induced nonalcoholic steatohepatitis and fibrosis in mice. Int. J. Biol. Sci. 2018, 14, 1411–1425. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Zheng, L.; Wu, J.; Mo, J.; Guo, L.; Wu, X.; Bao, Y. Polydatin Inhibits adipose tissue inflammation and ameliorates lipid metabolism in high-fat-fed mice. BioMed Res. Int. 2019, 2019, 7196535. [Google Scholar] [CrossRef] [PubMed]
- Li, L.; Tan, H.P.; Liu, C.Y.; Yu, L.T.; Wei, D.N.; Zhang, Z.C.; Lu, K.; Zhao, K.S.; Maegele, M.; Cai, D.Z.; et al. Polydatin prevents the induction of secondary brain injury after traumatic brain injury by protecting neuronal mitochondria. Neural. Regen. Res. 2019, 14, 1573–1582. [Google Scholar] [CrossRef]
- Yang, Q.B.; He, Y.L.; Zhong, X.W.; Xie, W.G.; Zhou, J.G. Resveratrol ameliorates gouty inflammation via upregulation of sirtuin 1 to promote autophagy in gout patients. Inflammopharmacology 2019, 27, 47–56. [Google Scholar] [CrossRef] [PubMed]
- Wang, J.; Chen, G.; Lu, L.; Zou, H. Sirt1 inhibits gouty arthritis via activating PPARγ. Clin. Rheumatol. 2019, 38, 3235–3242. [Google Scholar] [CrossRef]
- Reber, L.L.; Marichal, T.; Sokolove, J.; Starkl, P.; Gaudenzio, N.; Iwakura, Y.; Karasuyama, H.; Schwartz, L.B.; Robinson, W.H.; Tsai, M.; et al. Contribution of mast cell-derived interleukin-1β to uric acid crystal-induced acute arthritis in mice. Arthritis Rheumatol. 2014, 66, 2881–2891. [Google Scholar] [CrossRef]
- McCarthy, G.M.; Dunne, A. Calcium crystal deposition diseases—Beyond gout. Nat. Rev. Rheumatol. 2018, 14, 592–602. [Google Scholar] [CrossRef]
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Share and Cite
Oliviero, F.; Galozzi, P.; Scanu, A.; Galuppini, F.; Lazzarin, V.; Brocco, S.; Ravagnan, G.; Sfriso, P.; Ramonda, R.; Spinella, P.; Punzi, L.; Pennelli, G.; Luisetto, R. Polydatin Prevents Calcium Pyrophosphate Crystal-Induced Arthritis in Mice. Nutrients 2021, 13, 929. https://doi.org/10.3390/nu13030929
Oliviero F, Galozzi P, Scanu A, Galuppini F, Lazzarin V, Brocco S, Ravagnan G, Sfriso P, Ramonda R, Spinella P, Punzi L, Pennelli G, Luisetto R. Polydatin Prevents Calcium Pyrophosphate Crystal-Induced Arthritis in Mice. Nutrients. 2021; 13(3):929. https://doi.org/10.3390/nu13030929
Chicago/Turabian StyleOliviero, Francesca, Paola Galozzi, Anna Scanu, Francesca Galuppini, Vanni Lazzarin, Silvia Brocco, Giampietro Ravagnan, Paolo Sfriso, Roberta Ramonda, Paolo Spinella, Leonardo Punzi, Gianmaria Pennelli, and Roberto Luisetto. 2021. "Polydatin Prevents Calcium Pyrophosphate Crystal-Induced Arthritis in Mice" Nutrients 13, no. 3: 929. https://doi.org/10.3390/nu13030929