Next Article in Journal
A Review of the Potential Interaction of Selenium and Iodine on Placental and Child Health
Previous Article in Journal
Correction: Breakfast in Canada: Prevalence of Consumption, Contribution to Nutrient and Food Group Intakes, and Variability across Tertiles of Daily Diet Quality. A Study from the International Breakfast Research Initiative. Nutrients 2018, 10, 985
Open AccessArticle

Dietary α-Linolenic Acid Counters Cardioprotective Dysfunction in Diabetic Mice: Unconventional PUFA Protection

1
School of Medical Science, Griffith University Gold Coast, Southport QLD 4217, Australia
2
VA San Diego Healthcare System and Department of Anesthesiology, University of California, San Diego, CA 92093, USA
*
Author to whom correspondence should be addressed.
Joint first authors.
Nutrients 2020, 12(9), 2679; https://doi.org/10.3390/nu12092679
Received: 21 July 2020 / Revised: 12 August 2020 / Accepted: 26 August 2020 / Published: 2 September 2020
(This article belongs to the Section Macronutrients and Human Health)
Whether dietary omega-3 (n-3) polyunsaturated fatty acid (PUFA) confers cardiac benefit in cardiometabolic disorders is unclear. We test whether dietary α-linolenic acid (ALA) enhances myocardial resistance to ischemia-reperfusion (I-R) and responses to ischemic preconditioning (IPC) in type 2 diabetes (T2D); and involvement of conventional PUFA-dependent mechanisms (caveolins/cavins, kinase signaling, mitochondrial function, and inflammation). Eight-week male C57Bl/6 mice received streptozotocin (75 mg/kg) and 21 weeks high-fat/high-carbohydrate feeding. Half received ALA over six weeks. Responses to I-R/IPC were assessed in perfused hearts. Localization and expression of caveolins/cavins, protein kinase B (AKT), and glycogen synthase kinase-3β (GSK3β); mitochondrial function; and inflammatory mediators were assessed. ALA reduced circulating leptin, without affecting body weight, glycemic dysfunction, or cholesterol. While I-R tolerance was unaltered, paradoxical injury with IPC was reversed to cardioprotection with ALA. However, post-ischemic apoptosis (nucleosome content) appeared unchanged. Benefit was not associated with shifts in localization or expression of caveolins/cavins, p-AKT, p-GSK3β, or mitochondrial function. Despite mixed inflammatory mediator changes, tumor necrosis factor-a (TNF-a) was markedly reduced. Data collectively reveal a novel impact of ALA on cardioprotective dysfunction in T2D mice, unrelated to caveolins/cavins, mitochondrial, or stress kinase modulation. Although evidence suggests inflammatory involvement, the basis of this “un-conventional” protection remains to be identified. View Full-Text
Keywords: α-linolenic acid; n-3 PUFA; diabetes; cardioprotection; caveolae; caveolins; cavins; inflammation; ischemia-reperfusion; mitochondria α-linolenic acid; n-3 PUFA; diabetes; cardioprotection; caveolae; caveolins; cavins; inflammation; ischemia-reperfusion; mitochondria
Show Figures

Graphical abstract

MDPI and ACS Style

Russell, J.S.; Griffith, T.A.; Naghipour, S.; Vider, J.; Du Toit, E.F.; Patel, H.H.; Peart, J.N.; Headrick, J.P. Dietary α-Linolenic Acid Counters Cardioprotective Dysfunction in Diabetic Mice: Unconventional PUFA Protection. Nutrients 2020, 12, 2679.

AMA Style

Russell JS, Griffith TA, Naghipour S, Vider J, Du Toit EF, Patel HH, Peart JN, Headrick JP. Dietary α-Linolenic Acid Counters Cardioprotective Dysfunction in Diabetic Mice: Unconventional PUFA Protection. Nutrients. 2020; 12(9):2679.

Chicago/Turabian Style

Russell, Jake S.; Griffith, Tia A.; Naghipour, Saba; Vider, Jelena; Du Toit, Eugene F.; Patel, Hemal H.; Peart, Jason N.; Headrick, John P. 2020. "Dietary α-Linolenic Acid Counters Cardioprotective Dysfunction in Diabetic Mice: Unconventional PUFA Protection" Nutrients 12, no. 9: 2679.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop