Inhibitory Effects of a Sargassum miyabei Yendo on Cutibacterium acnes-Induced Skin Inflammation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Preparation of a Sargassum Miyabei Yendo Ethanol Extract
2.3. Bacterial Strains and Culture Conditions
2.4. Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentration (MBC)
2.5. C. acnes-Induced Inflammation in Mice
2.6. Histopathology
2.7. Immunohistochemistry
2.8. Cell Culture
2.9. Cell Viability
2.10. Enzyme-Linked Immunosorbent Assay
2.11. Western Blotting
2.12. Cell Migration
2.13. Electrophoretic Mobility Shift Assay
2.14. Statistical Analysis
3. Results
3.1. Antibacterial Effects of SMYEE
3.2. Effects of SMYEE on C. acnes-Induced Clinical Changes
3.3. SMYEE Suppressed Neutrophil Accumulation in Inflammatory Lesions
3.4. Effect of SMYEE on HaCaT Cell Viability
3.5. Effects of SMYEE on IL-8 Levels in C. acnes-Stimulated HaCaT Cells
3.6. Effects of SMYEE on dHL-60 Cell Migration
3.7. Effects of SMYEE on Extracellular Signal-Regulated Kinase (ERK) Signaling Pathways in C. acnes-Stimulated HaCaT Cells
3.8. Effects of SMYEE on Activator Protein-1 (AP-1) Activation in C. acnes-Stimulated HaCaT Cells
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Strains | MIC (µg/mL) | MBC (µg/mL) |
---|---|---|
C. acnes KCTC 3314 | 256 | 512 |
C. acnes isolates 2874 | 256 | 2048 |
C. acnes isolates 2875 | 256 | 1024 |
C. acnes isolates 2876 | 128 | 1024 |
C. acnes isolates 2878 | 512 | 4096 |
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Yim, M.-J.; Lee, J.M.; Kim, H.-S.; Choi, G.; Kim, Y.-M.; Lee, D.-S.; Choi, I.-W. Inhibitory Effects of a Sargassum miyabei Yendo on Cutibacterium acnes-Induced Skin Inflammation. Nutrients 2020, 12, 2620. https://doi.org/10.3390/nu12092620
Yim M-J, Lee JM, Kim H-S, Choi G, Kim Y-M, Lee D-S, Choi I-W. Inhibitory Effects of a Sargassum miyabei Yendo on Cutibacterium acnes-Induced Skin Inflammation. Nutrients. 2020; 12(9):2620. https://doi.org/10.3390/nu12092620
Chicago/Turabian StyleYim, Mi-Jin, Jeong Min Lee, Hyun-Soo Kim, Grace Choi, Young-Mog Kim, Dae-Sung Lee, and Il-Whan Choi. 2020. "Inhibitory Effects of a Sargassum miyabei Yendo on Cutibacterium acnes-Induced Skin Inflammation" Nutrients 12, no. 9: 2620. https://doi.org/10.3390/nu12092620