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Nutrients
Volume 12
Issue 8
10.3390/nu12082251
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Article
Peer-Review Record

Barrier Protection and Recovery Effects of Gut Commensal Bacteria on Differentiated Intestinal Epithelial Cells In Vitro

Nutrients 2020, 12(8), 2251; https://doi.org/10.3390/nu12082251
by Nooshin Mohebali, Katharina Ekat, Bernd Kreikemeyer and Anne Breitrück *,†
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Nutrients 2020, 12(8), 2251; https://doi.org/10.3390/nu12082251
Submission received: 26 June 2020 / Revised: 21 July 2020 / Accepted: 22 July 2020 / Published: 28 July 2020
(This article belongs to the Special Issue Role of Prebiotics and Probiotics in Health and Disease)

Round 1

Reviewer 1 Report

The manuscript reported the effects of 3 commensal bacteria in the human gut on inflammation in vitro. It is an interesting connection to clarify bacteria-host interactions that would compile the available knowledge about the overall health. The manuscript is well-written and only a few minor issues that the authors need to address.

L55, L79, L441, L483, L484: Cite a reference

L220: Clarify when do you decide to choose one analysis than the other

L456: Delete “significantly”

L459: Add the exact percentage

L491: Define the direction of the alterations? Increased or decreased

L518: Gene not gen

L544: “in vitro” should be italic

 

 

 

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Reviewer 2 Report

Review manuscript described by Mohebali et al. “Barrier Protection and Recovery Effects of Gut Commensal Bacteria on differentiated Intestinal Epithelial Cells in Vitro” is very interesting to me and significant especially in relation to host-microbial interaction in various inflammatory bowel diseases. There is some suggestions need to be addressed:

 

Major comments:

  1. The technique and methodology used for the co-culture of human intestinal cells Caco-2 and HT29-MTX cells with anaerobic bacteria is not appropriate to show host-microbial interaction. As, transwell inserts do not provide a vascular interface nor can they sustain luminal oxygen levels below 0.5%, which is required for co-culture of obligate anaerobes. Another major limitation is that in the medium in the dual-oxygen environment, the oxygenation level of the epithelial cells is not known, raising the question whether the cells are hypoxic. The methods like HoxBan system, organoid culture etc. could be implemented to prove these results.
  2. Culture of mammalian intestinal cells under hypoxic condition leads to activation of Hypoxia-Inducible Factor(HIF)-1 and its subsequent accumulation within the cells. Author need to address this issue with the current experiment in relation to host-microbial interaction.

 

Minor comments:

Scale bar for figure 5-C need to be provided.

Author Response

Please see the attachment.

Author Response File: Author Response.docx

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