Next Article in Journal
Asaronic Acid Inhibited Glucose-Triggered M2-Phenotype Shift Through Disrupting the Formation of Coordinated Signaling of IL-4Rα-Tyk2-STAT6 and GLUT1-Akt-mTOR-AMPK
Previous Article in Journal
Impact of a Ketogenic Diet on Metabolic Parameters in Patients with Obesity or Overweight and with or without Type 2 Diabetes: A Meta-Analysis of Randomized Controlled Trials
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Nutrients
Volume 12
Issue 7
10.3390/nu12072007
nutrients-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Structure and Function of Bovine Whey Derived Oligosaccharides Showing Synbiotic Epithelial Barrier Protective Properties

by
Peter I. Duncan
1,
Olli Aitio
2,
Annamari Heiskanen
2,
Ritva Niemelä
2,
Juhani Saarinen
2,
Jari Helin
2,
Nadine Porta
1,
Muriel Fiaux
1,
Denis Moënnoz
1,†,
Mireille Golliard
1,
Christine Cherbut
1,‡,
Rafael Berrocal
3,
Sean Austin
4 and
Norbert Sprenger
1,*
1
Nestlé Institute of Health Sciences, Société des Produits Nestlé S.A., 1000 Lausanne, Switzerland
2
Glykos Finland Ltd., 00790 Helsinki, Finland
3
Nestlé Product Technology Center, Société des Produits Nestlé S.A., 3510 Konolfingen, Switzerland
4
Nestlé Institute of Food Safety and Analytical Sciences, Société des Produits Nestlé S.A., 1000 Lausanne, Switzerland
*
Author to whom correspondence should be addressed.
†
This author had passed away.
‡
Current address: INRA, 75007 Paris, France.
Nutrients 2020, 12(7), 2007; https://doi.org/10.3390/nu12072007
Submission received: 4 June 2020 / Revised: 29 June 2020 / Accepted: 30 June 2020 / Published: 6 July 2020
(This article belongs to the Section Prebiotics, Probiotics and Postbiotics)
Browse Figures
Versions Notes

Abstract

Commensal gut microbiota and probiotics have numerous effects on the host’s metabolic and protective systems, which occur primarily through the intestinal epithelial cell interface. Prebiotics, like galacto-oligosaccharides (GOS) are widely used to modulate their function and abundance. However, important structure–function relations may exist, requiring a detailed structural characterization. Here, we detailed the structural characterization of bovine whey derived oligosaccharide preparations enriched with GOS or not, dubbed GOS-enriched milk oligosaccharides (GMOS) or MOS, respectively. We explore GMOS’s and MOS’s potential to improve intestinal epithelial barrier function, assessed in a model based on barrier disruptive effects of the Clostridioides difficile toxin A. GMOS and MOS contain mainly GOS species composed of β1-6- and β1-3-linked galactoses, and 3′- and 6′-sialyllactose. Both GMOS and MOS, combined with lactobacilli, like Lactobacillus rhamnosus (LPR, NCC4007), gave synergistic epithelial barrier protection, while no such effect was observed with Bifidobacterium longum (BL NCC3001), Escherichia coli (Nissle) or fructo-oligosaccharides. Mechanistically, for barrier protection with MOS, (i) viable LPR was required, (ii) acidification of growth medium was not enough, (iii) LPR did not directly neutralize toxin A, and (iv) physical proximity of LPR with the intestinal epithelial cells was necessary. This is the first study, highlighting the importance of structure–function specificity and the necessity of the simultaneous presence of prebiotic, probiotic and host cell interactions required for a biological effect.
Keywords: epithelial barrier function; lactobacillus probiotic; host–microbiota interaction epithelial barrier function; lactobacillus probiotic; host–microbiota interaction

Share and Cite

MDPI and ACS Style

Duncan, P.I.; Aitio, O.; Heiskanen, A.; Niemelä, R.; Saarinen, J.; Helin, J.; Porta, N.; Fiaux, M.; Moënnoz, D.; Golliard, M.; et al. Structure and Function of Bovine Whey Derived Oligosaccharides Showing Synbiotic Epithelial Barrier Protective Properties. Nutrients 2020, 12, 2007. https://doi.org/10.3390/nu12072007

AMA Style

Duncan PI, Aitio O, Heiskanen A, Niemelä R, Saarinen J, Helin J, Porta N, Fiaux M, Moënnoz D, Golliard M, et al. Structure and Function of Bovine Whey Derived Oligosaccharides Showing Synbiotic Epithelial Barrier Protective Properties. Nutrients. 2020; 12(7):2007. https://doi.org/10.3390/nu12072007

Chicago/Turabian Style

Duncan, Peter I., Olli Aitio, Annamari Heiskanen, Ritva Niemelä, Juhani Saarinen, Jari Helin, Nadine Porta, Muriel Fiaux, Denis Moënnoz, Mireille Golliard, and et al. 2020. "Structure and Function of Bovine Whey Derived Oligosaccharides Showing Synbiotic Epithelial Barrier Protective Properties" Nutrients 12, no. 7: 2007. https://doi.org/10.3390/nu12072007

APA Style

Duncan, P. I., Aitio, O., Heiskanen, A., Niemelä, R., Saarinen, J., Helin, J., Porta, N., Fiaux, M., Moënnoz, D., Golliard, M., Cherbut, C., Berrocal, R., Austin, S., & Sprenger, N. (2020). Structure and Function of Bovine Whey Derived Oligosaccharides Showing Synbiotic Epithelial Barrier Protective Properties. Nutrients, 12(7), 2007. https://doi.org/10.3390/nu12072007

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop