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Dietary Supplementation with Dunaliella Tertiolecta Prevents Whitening of Brown Fat and Controls Diet-Induced Obesity at Thermoneutrality in Mice

1
Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, Kasugai 487-8501, Japan
2
MAC Gifu Research Institute, MicroAlgae Corporation, 4-15 Akebono, Gifu 500-8148, Japan
*
Author to whom correspondence should be addressed.
Nutrients 2020, 12(6), 1686; https://doi.org/10.3390/nu12061686
Received: 14 May 2020 / Revised: 1 June 2020 / Accepted: 2 June 2020 / Published: 5 June 2020
(This article belongs to the Section Phytochemicals and Human Health)
We investigated the effect of evodiamine-containing microalga Dunaliella tertiolecta (DT) on the prevention of diet-induced obesity in a thermoneutral C57BL/6J male (30 °C). It attenuates the activity of brown adipose tissue (BAT), which accelerates diet-induced obesity. Nine-week-old mice were fed a high-fat diet supplemented with 10 g (Low group) or 25 g (High group) DT powder per kg food for 12 weeks. Compared to control mice without DT supplementation, body weight gain was significantly reduced in the High group with no difference in food intake. Tissue analyses indicated maintenance of multilocular morphology in BAT and reduced fat deposition in liver in DT-supplemented mice. Molecular analysis showed a significant decrease in mammalian target of rapamycin−ribosomal S6 protein kinase signaling pathway in white adipose tissue and upregulation in mRNA expression of brown fat-associated genes including fibroblast growth factor-21 (Fgf21) and uncoupling protein 1 (Ucp1) in BAT in the High group compared to the control. In the experiments using C3H10T1/2 adipocytes, DT extract upregulated mRNA expression of brown fat-associated genes in dose-dependent and time-dependent manners, accompanied by a significant increase in secreted FGF21 levels. Our data show the ability of DT as a nutraceutical to prevent brown fat attenuation and diet-induced obesity in vivo. View Full-Text
Keywords: brown fat; fibroblast growth factor-21; microalga; obesity; thermoneutrality; uncoupling protein 1 brown fat; fibroblast growth factor-21; microalga; obesity; thermoneutrality; uncoupling protein 1
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MDPI and ACS Style

Yamashita, Y.; Takeuchi, T.; Endo, Y.; Goto, A.; Sakaki, S.; Yamaguchi, Y.; Takenaka, H.; Yamashita, H. Dietary Supplementation with Dunaliella Tertiolecta Prevents Whitening of Brown Fat and Controls Diet-Induced Obesity at Thermoneutrality in Mice. Nutrients 2020, 12, 1686. https://doi.org/10.3390/nu12061686

AMA Style

Yamashita Y, Takeuchi T, Endo Y, Goto A, Sakaki S, Yamaguchi Y, Takenaka H, Yamashita H. Dietary Supplementation with Dunaliella Tertiolecta Prevents Whitening of Brown Fat and Controls Diet-Induced Obesity at Thermoneutrality in Mice. Nutrients. 2020; 12(6):1686. https://doi.org/10.3390/nu12061686

Chicago/Turabian Style

Yamashita, Yukari, Tamaki Takeuchi, Yuki Endo, Ayumi Goto, Setsuko Sakaki, Yuji Yamaguchi, Hiroyuki Takenaka, and Hitoshi Yamashita. 2020. "Dietary Supplementation with Dunaliella Tertiolecta Prevents Whitening of Brown Fat and Controls Diet-Induced Obesity at Thermoneutrality in Mice" Nutrients 12, no. 6: 1686. https://doi.org/10.3390/nu12061686

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