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Open AccessArticle

Effects of Standardized Green Tea Extract and Its Main Component, EGCG, on Mitochondrial Function and Contractile Performance of Healthy Rat Cardiomyocytes

1
Department of Chemistry, Life Sciences and Environmental Sustainability (SCVSA), University of Parma, 43124 Parma, Italy
2
Department of Biomedical and Neuromotor Sciences (DIBINEM), Laboratory of Biochemistry and Mitochondrial Pathophysiology, University of Bologna, 40126 Bologna, Italy
3
Department of Medicine and Surgery (DIMEC), University of Parma, 43125 Parma, Italy
4
National Institute of Biostructure and Biosystems (INBB), 00136 Rome, Italy
5
Centre for Molecular and Translational Oncology (COMT), University of Parma, 43124 Parma, Italy
6
CNR Institute of Molecular Genetics “Luigi Luca Cavalli-Sforza” Unit of Bologna, 40136 Bologna, Italy
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Nutrients 2020, 12(10), 2949; https://doi.org/10.3390/nu12102949
Received: 31 July 2020 / Revised: 23 September 2020 / Accepted: 23 September 2020 / Published: 25 September 2020
(This article belongs to the Special Issue Health-Promoting Components of Foods in Human Health)
We recently showed that the long-term in vivo administration of green tea catechin extract (GTE) resulted in hyperdynamic cardiomyocyte contractility. The present study investigates the mechanisms underlying GTE action in comparison to its major component, epigallocatechin-3-gallate (EGCG), given at the equivalent amount that would be in the entirety of GTE. Twenty-six male Wistar rats were given 40 mL/day of a tap water solution with either standardized GTE or pure EGCG for 4 weeks. Cardiomyocytes were then isolated for the study. Cellular bioenergetics was found to be significantly improved in both GTE- and EGCG-fed rats compared to that in controls as shown by measuring the maximal mitochondrial respiration rate and the cellular ATP level. Notably, the improvement of mitochondrial function was associated with increased levels of oxidative phosphorylation complexes, whereas the cellular mitochondrial mass was unchanged. However, only the GTE supplement improved cardiomyocyte mechanics and intracellular calcium dynamics, by lowering the expression of total phospholamban (PLB), which led to an increase of both the phosphorylated-PLB/PLB and the sarco-endoplasmic reticulum calcium ATPase/PLB ratios. Our findings suggest that GTE might be a valuable adjuvant tool for counteracting the occurrence and/or the progression of cardiomyopathies in which mitochondrial dysfunction and alteration of intracellular calcium dynamics constitute early pathogenic factors. View Full-Text
Keywords: cardiomyocyte mechanics; green tea extracts; mitochondria; oxidative phosphorylation; phospholamban; SERCA2 cardiomyocyte mechanics; green tea extracts; mitochondria; oxidative phosphorylation; phospholamban; SERCA2
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MDPI and ACS Style

Vilella, R.; Sgarbi, G.; Naponelli, V.; Savi, M.; Bocchi, L.; Liuzzi, F.; Righetti, R.; Quaini, F.; Frati, C.; Bettuzzi, S.; Solaini, G.; Stilli, D.; Rizzi, F.; Baracca, A. Effects of Standardized Green Tea Extract and Its Main Component, EGCG, on Mitochondrial Function and Contractile Performance of Healthy Rat Cardiomyocytes. Nutrients 2020, 12, 2949. https://doi.org/10.3390/nu12102949

AMA Style

Vilella R, Sgarbi G, Naponelli V, Savi M, Bocchi L, Liuzzi F, Righetti R, Quaini F, Frati C, Bettuzzi S, Solaini G, Stilli D, Rizzi F, Baracca A. Effects of Standardized Green Tea Extract and Its Main Component, EGCG, on Mitochondrial Function and Contractile Performance of Healthy Rat Cardiomyocytes. Nutrients. 2020; 12(10):2949. https://doi.org/10.3390/nu12102949

Chicago/Turabian Style

Vilella, Rocchina; Sgarbi, Gianluca; Naponelli, Valeria; Savi, Monia; Bocchi, Leonardo; Liuzzi, Francesca; Righetti, Riccardo; Quaini, Federico; Frati, Caterina; Bettuzzi, Saverio; Solaini, Giancarlo; Stilli, Donatella; Rizzi, Federica; Baracca, Alessandra. 2020. "Effects of Standardized Green Tea Extract and Its Main Component, EGCG, on Mitochondrial Function and Contractile Performance of Healthy Rat Cardiomyocytes" Nutrients 12, no. 10: 2949. https://doi.org/10.3390/nu12102949

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