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Open AccessArticle

Calorie Restriction Modulates Reproductive Development and Energy Balance in Pre-Pubertal Male Rats

1
Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary 3330 Hospital Dr. NW, Calgary, AB T2N 4N1, Canada
2
Gastrointestinal Research Group, Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB T2N 4N1, Canada
*
Author to whom correspondence should be addressed.
Nutrients 2019, 11(9), 1993; https://doi.org/10.3390/nu11091993
Received: 3 July 2019 / Revised: 14 August 2019 / Accepted: 19 August 2019 / Published: 23 August 2019
The objective was to determine effects of feed restriction and refeeding on reproductive development and energy balance in pre-pubertal male rats. Sprague Dawley rats (n = 32, 24 days old, ~65 g), were randomly allocated into four treatments (n = 8/treatment): (1) Control (CON, ad libitum feed; (2) Mild Restriction (MR, rats fed 75% of CON consumption); (3) Profound Restriction (PR, 50% of CON consumption); or (4) Refeeding (RF, 50% restriction for 14 days, and then ad libitum for 7 days). Feed restriction delayed reproductive development and decreased energy balance and tissue accretion, with degree of reproductive and metabolic dysfunctions related to restriction severity. In RF rats, refeeding largely restored testis weight, sperm production (per gram and total), plasma IGF-1, leptin and insulin concentrations and energy expenditure, although body composition did not completely recover. On Day 50, more CON and RF rats than PR rats were pubertal (5/6, 4/5 and 1/6, respectively; plasma testosterone >1 ng/mL) with the MR group (4/6) not different. Our hypothesis was supported: nutrient restriction of pre-pubertal rats delayed reproductive development, induced negative energy balance and decreased metabolic hormone concentrations (commensurate with restriction), whereas short-term refeeding after profound restriction largely restored reproductive end points and plasma hormone concentrations, but not body composition. View Full-Text
Keywords: caloric restriction; restricted feeding; realimentation; puberty; reproductive development caloric restriction; restricted feeding; realimentation; puberty; reproductive development
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Rizzoto, G.; Sekhar, D.; Thundathil, J.C.; Chelikani, P.K.; Kastelic, J.P. Calorie Restriction Modulates Reproductive Development and Energy Balance in Pre-Pubertal Male Rats. Nutrients 2019, 11, 1993.

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