Serine Phosphorylation of IRS1 Correlates with Aβ-Unrelated Memory Deficits and Elevation in Aβ Level Prior to the Onset of Memory Decline in AD
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Western Blotting
2.3. Water T Maze Test
2.4. Measurement of Metabolic Parameters
2.5. Enzyme-Linked Immunosorbent Assay (ELISA) Quantitation of Aβ
2.6. Statistics
3. Results
3.1. Activation of Specific Ser Residues on Hippocampal Insulin Receptor Substrate 1 (IRS1) Is Associated with T2DM-Induced Memory Impairment
3.2. Type 1 Diabetes Mellitus (T1DM)-Induced Memory Deficits Occur Independently of IRS1 Activity
3.3. Phosphorylation of IRS1 at Age-Specific Ser Residues with the Activation of Downstream Kinases Is Linked to Age-Related Memory Deficits
3.4. Increased Phosphorylation of Hippocampal IRS1 at Ser Residues in Young Amyloid Precursor Protein (APP) Knock-In (APP KINL-G-F) Mice Occurs Prior to the Onset of Memory Decline
3.5. Memory Decline in Middle-Aged APPKINL-G-F Mice Is Accompanied by the Activation of Specific Ser Sites on IRS1 and Energy Depletion
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Wang, W.; Tanokashira, D.; Fukui, Y.; Maruyama, M.; Kuroiwa, C.; Saito, T.; Saido, T.C.; Taguchi, A. Serine Phosphorylation of IRS1 Correlates with Aβ-Unrelated Memory Deficits and Elevation in Aβ Level Prior to the Onset of Memory Decline in AD. Nutrients 2019, 11, 1942. https://doi.org/10.3390/nu11081942
Wang W, Tanokashira D, Fukui Y, Maruyama M, Kuroiwa C, Saito T, Saido TC, Taguchi A. Serine Phosphorylation of IRS1 Correlates with Aβ-Unrelated Memory Deficits and Elevation in Aβ Level Prior to the Onset of Memory Decline in AD. Nutrients. 2019; 11(8):1942. https://doi.org/10.3390/nu11081942
Chicago/Turabian StyleWang, Wei, Daisuke Tanokashira, Yusuke Fukui, Megumi Maruyama, Chiemi Kuroiwa, Takashi Saito, Takaomi C. Saido, and Akiko Taguchi. 2019. "Serine Phosphorylation of IRS1 Correlates with Aβ-Unrelated Memory Deficits and Elevation in Aβ Level Prior to the Onset of Memory Decline in AD" Nutrients 11, no. 8: 1942. https://doi.org/10.3390/nu11081942
APA StyleWang, W., Tanokashira, D., Fukui, Y., Maruyama, M., Kuroiwa, C., Saito, T., Saido, T. C., & Taguchi, A. (2019). Serine Phosphorylation of IRS1 Correlates with Aβ-Unrelated Memory Deficits and Elevation in Aβ Level Prior to the Onset of Memory Decline in AD. Nutrients, 11(8), 1942. https://doi.org/10.3390/nu11081942