Sex-Specific Differences in Fat Storage, Development of Non-Alcoholic Fatty Liver Disease and Brain Structure in Juvenile HFD-Induced Obese Ldlr-/-.Leiden Mice
Sophie A.H. Jacobs 1
, Eveline Gart 2,3, Debby Vreeken 1, Bart A.A. Franx 1, Lotte Wekking 1, Vivienne G.M. Verweij 1, Nicole Worms 2, Marieke H. Schoemaker 4, Gabriele Gross 4, Martine C. Morrison 2,3, Robert Kleemann 2,5, Ilse A.C. Arnoldussen 1,† and Amanda J. Kiliaan 1,*,†
, Eveline Gart 2,3, Debby Vreeken 1, Bart A.A. Franx 1, Lotte Wekking 1, Vivienne G.M. Verweij 1, Nicole Worms 2, Marieke H. Schoemaker 4, Gabriele Gross 4, Martine C. Morrison 2,3, Robert Kleemann 2,5, Ilse A.C. Arnoldussen 1,† and Amanda J. Kiliaan 1,*,†
1
Department of Anatomy, Radboud university medical center, Donders Institute for Brain, Cognition and Behaviour, Preclinical Imaging Center PRIME, 6500 HB Nijmegen, The Netherlands
2
Department of Metabolic Health Research, Netherlands Organisation for Applied Scientific Research (TNO), 2333 CK Leiden, The Netherlands
3
Human and Animal Physiology, Wageningen University, 6700 AH Wageningen, The Netherlands
4
Reckitt Benckiser Health, Mead Johnson Pediatric Nutrition Institute, 6545 CJ Nijmegen, The Netherlands
5
Department of Vascular Surgery, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to the work.
Nutrients 2019, 11(8), 1861; https://doi.org/10.3390/nu11081861
Received: 24 June 2019 / Revised: 1 August 2019 / Accepted: 7 August 2019 / Published: 10 August 2019
(This article belongs to the Special Issue Diet, Lipid and Lipoprotein Metabolism and Human Health)
Abstract
Background: Sex-specific differences play a role in metabolism, fat storage in adipose tissue, and brain structure. At juvenile age, brain function is susceptible to the effects of obesity; little is known about sex-specific differences in juvenile obesity. Therefore, this study examined sex-specific differences in adipose tissue and liver of high-fat diet (HFD)-induced obese mice, and putative alterations between male and female mice in brain structure in relation to behavioral changes during the development of juvenile obesity. Methods: In six-week-old male and female Ldlr-/-.Leiden mice (n = 48), the impact of 18 weeks of HFD-feeding was examined. Fat distribution, liver pathology and brain structure and function were analyzed imunohisto- and biochemically, in cognitive tasks and with MRI. Results: HFD-fed female mice were characterized by an increased perigonadal fat mass, pronounced macrovesicular hepatic steatosis and liver inflammation. Male mice on HFD displayed an increased mesenteric fat mass, pronounced adipose tissue inflammation and microvesicular hepatic steatosis. Only male HFD-fed mice showed decreased cerebral blood flow and reduced white matter integrity. Conclusions: At young age, male mice are more susceptible to the detrimental effects of HFD than female mice. This study emphasizes the importance of sex-specific differences in obesity, liver pathology, and brain function. View Full-Text
Graphical abstract
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
Share & Cite This Article
MDPI and ACS Style
Jacobs, S.A.; Gart, E.; Vreeken, D.; Franx, B.A.; Wekking, L.; Verweij, V.G.; Worms, N.; Schoemaker, M.H.; Gross, G.; Morrison, M.C.; Kleemann, R.; Arnoldussen, I.A.; Kiliaan, A.J. Sex-Specific Differences in Fat Storage, Development of Non-Alcoholic Fatty Liver Disease and Brain Structure in Juvenile HFD-Induced Obese Ldlr-/-.Leiden Mice. Nutrients 2019, 11, 1861.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
Related Articles
Article Metrics
Comments
[Return to top]
Nutrients
EISSN 2072-6643
Published by MDPI AG, Basel, Switzerland
RSS
E-Mail Table of Contents Alert