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Human Enriched Serum Following Hydrolysed Collagen Absorption Modulates Bone Cell Activity: from Bedside to Bench and Vice Versa

INRA, UMR 1019, UNH, CRNH Auvergne, F-63009 Clermont-Ferrand, France
Unité de Nutrition Humaine, Clermont Université, Université d’Auvergne, BP 10448, F-63000 Clermont-Ferrand, France
Rousselot BVBA, Meulestedekaai 81, 9000 Gent, Belgium
Rousselot SAS, 4 rue de l’abreuvoir, 92400 Courbevoie, France
CRB Auvergne, Hématologie Biologique, Equipe d’Accueil 7453 CHELTER, Centre Hospitalier Universitaire Estaing, 1 place Lucie et Raymond Aubrac, CEDEX 1, F-63003 Clermont-Ferrand, France
Biostatistics Unit (DRCI), University hospital Clermont-Ferrand, 58 rue Montalembert, 63000 Clermont-Ferrand, France
Inserm, UMR 1229, RMeS, Regenerative Medicine and Skeleton, Université de Nantes, ONIRIS, 44000 Nantes, France
UFR Odontologie, Université de Nantes, 44000 Nantes, France
CHU Nantes, PHU4 OTONN, 44000 Nantes, France
Chu Clermont-Ferrand, Centre De Recherche En Nutrition Humaine Auvergne, 58 rue Montalembert, 63000 Clermont-Ferrand, France
Author to whom correspondence should be addressed.
Nutrients 2019, 11(6), 1249;
Received: 24 April 2019 / Revised: 27 May 2019 / Accepted: 28 May 2019 / Published: 31 May 2019
PDF [1965 KB, uploaded 22 June 2019]


Collagen proteins are crucial components of the bone matrix. Since collagen-derived products are widely used in the food and supplement industry, one may raise the question whether collagen-enriched diets can provide benefits for the skeleton. In this study, we designed an innovative approach to investigate this question taking into account the metabolites that are formed by the digestive tract and appear in the circulation after ingestion of hydrolysed collagen. Blood samples collected in clinical and pre-clinical trials following ingestion and absorption of hydrolysed collagen were processed and applied on bone-related primary cell cultures. This original ex vivo methodology revealed that hydrolysed collagen-enriched serum had a direct impact on the behaviour of cells from both human and mouse origin that was not observed with controls (bovine serum albumin or hydrolysed casein-enriched serum). These ex vivo findings were fully in line with in vivo results obtained from a mouse model of post-menopausal osteoporosis. A significant reduction of bone loss was observed in mice supplemented with hydrolysed collagen compared to a control protein. Both the modulation of osteoblast and osteoclast activity observed upon incubation with human or mouse serum ex vivo and the attenuation of bone loss in vivo, clearly indicates that the benefits of hydrolysed collagen for osteoporosis prevention go beyond the effect of a simple protein supplementation. View Full-Text
Keywords: metabolites; bone; hydrolysed collagen; nutrition; osteoporosis; absorption; collagen peptides metabolites; bone; hydrolysed collagen; nutrition; osteoporosis; absorption; collagen peptides

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Wauquier, F.; Daneault, A.; Granel, H.; Prawitt, J.; Fabien Soulé, V.; Berger, J.; Pereira, B.; Guicheux, J.; Rochefort, G.Y.; Meunier, N.; Blot, A.; Wittrant, Y. Human Enriched Serum Following Hydrolysed Collagen Absorption Modulates Bone Cell Activity: from Bedside to Bench and Vice Versa. Nutrients 2019, 11, 1249.

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