Human Enriched Serum Following Hydrolysed Collagen Absorption Modulates Bone Cell Activity: from Bedside to Bench and Vice Versa
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics
2.2. Hydrolysed Collagens
2.3. Cell Cultures
2.4. Dietary Supplementation and Ovariectomy-Related Bone Loss
2.5. Tissue Sampling, Biochemical Parameters and Bone Mineral Density Analysis
2.6. Metabolism Models and Serum Collection
2.7. In Vitro and Ex Vivo Assays
2.8. Statistical Analysis
3. Results
3.1. Bovine HC Promotes Osteoblast Proliferation, Differentiation and Function in Vitro
3.2. B2000 Significantly Reduces Bone Loss in Vivo by Modulating the Level of RANKL
3.3. B2000-Enriched Mouse Serum Stimulates Osteoblast Function While Repressing Osteoclastogenesis
3.4. Assays with Human Enriched Sera Confirm the in Vivo Data and Allow Comparative Activity Screening.
4. Discussion
5. Conclusions
6. Patents
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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CTRL (RQ) | CASEIN (RQ) | CASEIN (p-Value) | B2000 (RQ) | B2000 (p-Value) | |
---|---|---|---|---|---|
Acp5 | 1.0 | 0.53 | 0.54 | 0.45 | 0.03 |
Car2 | 1.0 | 0.52 | 0.32 | 0.52 | 0.02 |
Csf1r | 1.0 | 0.57 | 0.03 | 0.78 | 0.02 |
Ctsk | 1.0 | 0.69 | 0.72 | 0.75 | 0.06 |
Itgb3 | 1.0 | 0.47 | 0.39 | 0.53 | 0.06 |
Mmp9 | 1.0 | 0.55 | 0.56 | 0.63 | 0.08 |
Nos2 | 1.0 | 0.47 | 0.15 | 0.24 | 0.06 |
Tgfbr1 | 1.0 | 0.43 | 0.37 | 0.34 | 0.06 |
Tnfrsf1b | 1.0 | 0.58 | 0.17 | 0.49 | 0.07 |
Traf2 | 1.0 | 0.56 | 0.12 | 0.69 | 0.06 |
Traf6 | 1.0 | 0.60 | 0.05 | 0.64 | 0.04 |
CASEIN (RQ) | B2000 (RQ) | B2000 (p-Value) | |
---|---|---|---|
Cd36 | 1.0 | 0.66 | 0.04 |
Itgam | 1.0 | 0.86 | 0.06 |
Tlr2 | 1.0 | 0.81 | 0.06 |
Tnfrsf1b | 1.0 | 0.77 | 0.03 |
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Wauquier, F.; Daneault, A.; Granel, H.; Prawitt, J.; Fabien Soulé, V.; Berger, J.; Pereira, B.; Guicheux, J.; Rochefort, G.Y.; Meunier, N.; et al. Human Enriched Serum Following Hydrolysed Collagen Absorption Modulates Bone Cell Activity: from Bedside to Bench and Vice Versa. Nutrients 2019, 11, 1249. https://doi.org/10.3390/nu11061249
Wauquier F, Daneault A, Granel H, Prawitt J, Fabien Soulé V, Berger J, Pereira B, Guicheux J, Rochefort GY, Meunier N, et al. Human Enriched Serum Following Hydrolysed Collagen Absorption Modulates Bone Cell Activity: from Bedside to Bench and Vice Versa. Nutrients. 2019; 11(6):1249. https://doi.org/10.3390/nu11061249
Chicago/Turabian StyleWauquier, Fabien, Audrey Daneault, Henri Granel, Janne Prawitt, Véronique Fabien Soulé, Juliette Berger, Bruno Pereira, Jérôme Guicheux, Gael Y. Rochefort, Nathalie Meunier, and et al. 2019. "Human Enriched Serum Following Hydrolysed Collagen Absorption Modulates Bone Cell Activity: from Bedside to Bench and Vice Versa" Nutrients 11, no. 6: 1249. https://doi.org/10.3390/nu11061249