Next Article in Journal
Association of Vegetarian Diet with Chronic Kidney Disease
Previous Article in Journal
Anti-Inflammatory Effect of Chloroform Fraction of Pyrus Ussuriensis Maxim. Leaf Extract on 2, 4-Dinitrochlorobenzene-Induced Atopic Dermatitis in nc/nga Mice
Open AccessArticle

3,5-Diiodo-L-Thyronine Exerts Metabolically Favorable Effects on Visceral Adipose Tissue of Rats Receiving a High-Fat Diet

1
Dipartimento di Scienze e Tecnologie, Università degli Studi del Sannio, via Port’Arsa 11, 82100 Benevento, Italy
2
Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Università‘ degli Studi della Campania Luigi Vanvitelli, via Vivaldi 43, 81100 Caserta, Italy
3
Dip. Scienze Biomolecolari, Salute, Università di Urbino “Carlo Bo”, via Maggetti, 26, 61029 Urbino, Italy
4
Dipartimento di Biologia, Università degli Studi di Napoli “Federico II”, via Cinthia, 80126 Napoli, Italy
5
Proteomics & Mass Spectrometry Laboratory, ISPAAM, National Research Council, via Argine, 1085, 80147 Naples, Italy
*
Authors to whom correspondence should be addressed.
Authors that contributed equally to this work.
Nutrients 2019, 11(2), 278; https://doi.org/10.3390/nu11020278
Received: 30 November 2018 / Revised: 21 January 2019 / Accepted: 24 January 2019 / Published: 27 January 2019
When administered to rats receiving a high-fat diet (HFD), 3,5-diiodo-L-thyronine (3,5-T2) [at a dose of 25 μg/100 g body weight (BW)] is known to increase energy expenditure and to prevent HFD-induced adiposity. Here, we investigated which cellular and molecular processes in visceral white adipose tissue (VAT) contributed to the beneficial effect of 3,5-T2 over time (between 1 day and 4 weeks following administration). 3,5-T2 programmed the adipocyte for lipolysis by rapidly inducing hormone sensitive lipase (HSL) phosphorylation at the protein kinase A-responsive site Ser563, accompanied with glycerol release at the 1-week time-point, contributing to the partial normalization of adipocyte volume with respect to control (N) animals. After two weeks, when the adipocyte volumes of HFD-3,5-T2 rats were completely normalized to those of the controls (N), 3,5-T2 consistently induced HSL phosphorylation at Ser563, indicative of a combined effect of 3,5-T2-induced adipose lipolysis and increasing non-adipose oxidative metabolism. VAT proteome analysis after 4 weeks of treatment revealed that 3,5-T2 significantly altered the proteomic profile of HFD rats and produced a marked pro-angiogenic action. This was associated with a reduced representation of proteins involved in lipid storage or related to response to oxidative stress, and a normalization of the levels of those involved in lipogenesis-associated mitochondrial function. In conclusion, the prevention of VAT mass-gain by 3,5-T2 occurred through different molecular pathways that, together with the previously reported stimulation of resting metabolism and liver fatty acid oxidation, are associated with an anti adipogenic/lipogenic potential and positively impact on tissue health. View Full-Text
Keywords: 3,5-diiodo-L-thyronine; visceral white adipose tissue; ATGL; hormone sensitive lipase; lipolysis; proteomics 3,5-diiodo-L-thyronine; visceral white adipose tissue; ATGL; hormone sensitive lipase; lipolysis; proteomics
Show Figures

Figure 1

MDPI and ACS Style

Silvestri, E.; Senese, R.; Cioffi, F.; De Matteis, R.; Lattanzi, D.; Lombardi, A.; Giacco, A.; Salzano, A.M.; Scaloni, A.; Ceccarelli, M.; Moreno, M.; Goglia, F.; Lanni, A.; de Lange, P. 3,5-Diiodo-L-Thyronine Exerts Metabolically Favorable Effects on Visceral Adipose Tissue of Rats Receiving a High-Fat Diet. Nutrients 2019, 11, 278.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop