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Chondroprotective Properties of Human-Enriched Serum Following Polyphenol Extract Absorption: Results from an Exploratory Clinical Trial

Clermont Auvergne University, INRA, UNH, 63000 Clermont-Ferrand, France
Inserm, UMR 1229, RMeS, Regenerative Medicine and Skeleton, Université de Nantes, ONIRIS, F-44042 Nantes, France
UFR Odontologie, Université de Nantes, F-44042 Nantes, France
UR Oenologie, Université de Bordeaux, ISVV, EA 4577, USC 1366 INRA, IPB4, F-33140 Villenave d’Ornon, France
INRAE, UMR 1019, UNH, 63122 Saint-Genès Champanelle, France
Grap’sud/Inosud, 120 chemin de la regor, 30360 Cruviers-Lascours, France
CRB Auvergne, Hématologie Biologique, Equipe d’Accueil 7453 CHELTER, CHU Estaing, 1 place Lucie et Raymond Aubrac, F-63003 Clermont-Ferrand, France
Orthopedics department, University Hospital Clermont-Ferrand, F-63003 Clermont-Ferrand, France
Rhumatology department, CHU Nantes, PHU4 OTONN, F-44042 Nantes, France
CHU Clermont-Ferrand, Centre de Recherche en Nutrition Humaine Auvergne, 58 rue Montalembert, F-63000 Clermont-Ferrand, France
Author to whom correspondence should be addressed.
Nutrients 2019, 11(12), 3071;
Received: 18 November 2019 / Revised: 6 December 2019 / Accepted: 12 December 2019 / Published: 16 December 2019
(This article belongs to the Section Micronutrients and Human Health)
Polyphenols are widely acknowledged for their health benefits, especially for the prevention of inflammatory and age-related diseases. We previously demonstrated that hydroxytyrosol (HT) and procyanidins (PCy), alone or in combination, drive preventive anti-osteoathritic effects in vivo. However, the lack of sufficient clinical evidences on the relationship between dietary phytochemicals and osteoarthritis remains. In this light, we investigated in humans the potential osteoarticular benefit of a grapeseed and olive extract (OPCO) characterized for its hydroxytyrosol (HT) and procyanidins (PCy) content. We first validated, in vitro, the anti-inflammatory and chondroprotective properties of the extract on primary cultured human articular chondrocytes stimulated by interleukin-1 beta (IL-1 β). The sparing effect involved a molecular mechanism dependent on the nuclear transcription factor-kappa B (NF-κB) pathway. To confirm the clinical relevance of such a nutritional strategy, we designed an innovative clinical approach taking into account the metabolites that are formed during the digestion process and that appear in circulation after the ingestion of the OPCO extract. Blood samples from volunteers were collected following ingestion, absorption, and metabolization of the extract and then were processed and applied on human primary chondrocyte cultures. This original ex vivo methodology confirmed at a clinical level the chondroprotective properties previously observed in vitro and in vivo. View Full-Text
Keywords: micronutrients; osteoarthritis (OA); clinical trials; hydroxytyrosol; procyanidins; cell biology micronutrients; osteoarthritis (OA); clinical trials; hydroxytyrosol; procyanidins; cell biology
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Wauquier, F.; Mevel, E.; Krisa, S.; Richard, T.; Valls, J.; Hornedo-Ortega, R.; Granel, H.; Boutin-Wittrant, L.; Urban, N.; Berger, J.; Descamps, S.; Guicheux, J.; Vinatier, C.S.; Beck, L.; Meunier, N.; Blot, A.; Wittrant, Y. Chondroprotective Properties of Human-Enriched Serum Following Polyphenol Extract Absorption: Results from an Exploratory Clinical Trial. Nutrients 2019, 11, 3071.

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