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Open AccessArticle

Impact of Vitamin D Deficit on the Rat Gut Microbiome

Department of Pharmacology, School of Pharmacy, Universidad de Granada, 18071 Granada, Spain
Ciber Enfermedades Cardiovasculares (CiberCV), 28029 Madrid, Spain
Department of Pharmacology and Toxicology, School of Medicine, Universidad Complutense de Madrid, 28040 Madrid, Spain
Ciber Enfermedades Respiratorias (Ciberes), 28029 Madrid, Spain
Instituto de Investigación Sanitaria Gregorio Marañón (IISGM), 28007 Madrid, Spain
Fundación Parque Científico de Madrid, 28049 Madrid, Spain
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Nutrients 2019, 11(11), 2564;
Received: 2 September 2019 / Revised: 14 October 2019 / Accepted: 16 October 2019 / Published: 24 October 2019
Inadequate immunologic, metabolic and cardiovascular homeostasis has been related to either an alteration of the gut microbiota or to vitamin D deficiency. We analyzed whether vitamin D deficiency alters rat gut microbiota. Male Wistar rats were fed a standard or a vitamin D-free diet for seven weeks. The microbiome composition was determined in fecal samples by 16S rRNA gene sequencing. The vitamin D-free diet produced mild changes on α- diversity but no effect on β-diversity in the global microbiome. Markers of gut dysbiosis like Firmicutes-to-Bacteroidetes ratio or the short chain fatty acid producing bacterial genera were not significantly affected by vitamin D deficiency. Notably, there was an increase in the relative abundance of the Enterobacteriaceae, with significant rises in its associated genera Escherichia, Candidatus blochmannia and Enterobacter in vitamin D deficient rats. Prevotella and Actinomyces were also increased and Odoribacteraceae and its genus Butyricimonas were decreased in rats with vitamin D-free diet. In conclusion, vitamin D deficit does not induce gut dysbiosis but produces some specific changes in bacterial taxa, which may play a pathophysiological role in the immunologic dysregulation associated with this hypovitaminosis. View Full-Text
Keywords: microbiota; 16S rRNA sequencing; vitamin D deficit microbiota; 16S rRNA sequencing; vitamin D deficit
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MDPI and ACS Style

Robles-Vera, I.; Callejo, M.; Ramos, R.; Duarte, J.; Perez-Vizcaino, F. Impact of Vitamin D Deficit on the Rat Gut Microbiome. Nutrients 2019, 11, 2564.

AMA Style

Robles-Vera I, Callejo M, Ramos R, Duarte J, Perez-Vizcaino F. Impact of Vitamin D Deficit on the Rat Gut Microbiome. Nutrients. 2019; 11(11):2564.

Chicago/Turabian Style

Robles-Vera, Iñaki; Callejo, María; Ramos, Ricardo; Duarte, Juan; Perez-Vizcaino, Francisco. 2019. "Impact of Vitamin D Deficit on the Rat Gut Microbiome" Nutrients 11, no. 11: 2564.

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