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Rotavirus Double Infection Model to Study Preventive Dietary Interventions

1
Departament de Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l’Alimentació, University of Barcelona (UB), Av. Joan XXIII 27-31, 08028 Barcelona, Spain
2
Institut de Recerca en Nutrició i Seguretat Alimentària (INSA), C/ Prat de la Riba 171, 08921 Santa Coloma de Gramanet, 08921 Barcelona, Spain
3
Nutricia Research, 3584 CT Utrecht, The Netherlands
4
Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584 CA Utrecht, The Netherlands
*
Author to whom correspondence should be addressed.
Nutrients 2019, 11(1), 131; https://doi.org/10.3390/nu11010131
Received: 4 December 2018 / Revised: 27 December 2018 / Accepted: 7 January 2019 / Published: 10 January 2019
(This article belongs to the Special Issue Nutritional Management of Diarrhea)
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Abstract

Rotaviruses are the main cause of acute diarrhea among young children worldwide with an increased frequency of reinfection. Several life style factors, such as dietary components, may influence such processes by affecting the outcome of the first rotavirus infection and therefore having a beneficial impact on the anti-rotavirus immune responses during any subsequent reinfections. The aim of this research was to develop a double-infection model in rat that mimics real-life clinical scenarios and would be useful in testing whether nutritional compounds can modulate the rotavirus-associated disease and immune response. Three experimental designs and a preventive dietary-like intervention were conducted in order to achieve a differential response in the double-infected animals compared to the single-infected ones and to study the potential action of a modulatory agent in early life. Diarrhea was only observed after the first infection, with a reduction of fecal pH and fever. After the second infection an increase in body temperature was also found. The immune response against the second infection was regulated by the preventive effect of the dietary-like intervention during the first infection in terms of specific antibodies and DTH. A rotavirus-double-infection rat model has been developed and is suitable for use in future preventive dietary intervention studies. View Full-Text
Keywords: rotavirus; diarrhea; rat; model; double-infection rotavirus; diarrhea; rat; model; double-infection
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Rigo-Adrover, M.M.; Knipping, K.; Garssen, J.; Saldaña-Ruíz, S.; Franch, À.; Castell, M.; Pérez-Cano, F.J. Rotavirus Double Infection Model to Study Preventive Dietary Interventions. Nutrients 2019, 11, 131.

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