Next Article in Journal
Assessing the Usability of the Automated Self-Administered Dietary Assessment Tool (ASA24) among Low-Income Adults
Previous Article in Journal
Plant-Based Diets for Cardiovascular Safety and Performance in Endurance Sports
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle

Rotavirus Double Infection Model to Study Preventive Dietary Interventions

Departament de Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l’Alimentació, University of Barcelona (UB), Av. Joan XXIII 27-31, 08028 Barcelona, Spain
Institut de Recerca en Nutrició i Seguretat Alimentària (INSA), C/ Prat de la Riba 171, 08921 Santa Coloma de Gramanet, 08921 Barcelona, Spain
Nutricia Research, 3584 CT Utrecht, The Netherlands
Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584 CA Utrecht, The Netherlands
Author to whom correspondence should be addressed.
Nutrients 2019, 11(1), 131;
Received: 4 December 2018 / Revised: 27 December 2018 / Accepted: 7 January 2019 / Published: 10 January 2019
(This article belongs to the Special Issue Nutritional Management of Diarrhea)
PDF [1321 KB, uploaded 10 January 2019]


Rotaviruses are the main cause of acute diarrhea among young children worldwide with an increased frequency of reinfection. Several life style factors, such as dietary components, may influence such processes by affecting the outcome of the first rotavirus infection and therefore having a beneficial impact on the anti-rotavirus immune responses during any subsequent reinfections. The aim of this research was to develop a double-infection model in rat that mimics real-life clinical scenarios and would be useful in testing whether nutritional compounds can modulate the rotavirus-associated disease and immune response. Three experimental designs and a preventive dietary-like intervention were conducted in order to achieve a differential response in the double-infected animals compared to the single-infected ones and to study the potential action of a modulatory agent in early life. Diarrhea was only observed after the first infection, with a reduction of fecal pH and fever. After the second infection an increase in body temperature was also found. The immune response against the second infection was regulated by the preventive effect of the dietary-like intervention during the first infection in terms of specific antibodies and DTH. A rotavirus-double-infection rat model has been developed and is suitable for use in future preventive dietary intervention studies. View Full-Text
Keywords: rotavirus; diarrhea; rat; model; double-infection rotavirus; diarrhea; rat; model; double-infection

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Rigo-Adrover, M.M.; Knipping, K.; Garssen, J.; Saldaña-Ruíz, S.; Franch, À.; Castell, M.; Pérez-Cano, F.J. Rotavirus Double Infection Model to Study Preventive Dietary Interventions. Nutrients 2019, 11, 131.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Nutrients EISSN 2072-6643 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top