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Open AccessArticle

Lead Affects Vitamin D Metabolism in Rats

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Department of Food Science and Nutrition, College of Life Sciences, Kuwait University, P.O. Box 5969, Safat 13060, Kuwait
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Department of Anatomy, Faculty of Medicine, Kuwait University, P.O. Box 24923, Safat 13110, Kuwait
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Author to whom correspondence should be addressed.
Nutrients 2018, 10(3), 264; https://doi.org/10.3390/nu10030264
Received: 4 January 2018 / Revised: 19 February 2018 / Accepted: 22 February 2018 / Published: 26 February 2018
A negative association between blood lead and vitamin D concentrations has been reported, however, experimental data on the effect of lead (Pb) on vitamin D metabolism is scarce. We investigated the effects of Pb on serum vitamin D metabolites, vitamin D activating enzymes and vitamin D receptor (VDR) in rats. Newborn Wistar rat pups were exposed to 0.2% Pb-acetate via their dams’ drinking water from post-natal day (PND) 1 to 21 and directly in drinking water until PND30. Serum 25-hydroxyvitamin D was analyzed with LC-MS/MS and 1,25-dihydroxyvitamin D with an immunoassay. Tissue expression of vitamin D activating enzymes and VDR were measured by Western blot and immunohistochemistry. Serum 25-hydroxyvitamin D was significantly decreased at both PND21 and PND30, whereas 1,25-dihydroxyvitamin D was decreased (p < 0.05) only at PND21 in the Pb-exposed rats. Expression of renal 1-α-hydroxylase was decreased by Pb only at PND21 (p < 0.05) but the brain 1-α-hydroxylase was not affected. Hepatic 25-hydroxylase expression was significantly decreased at PND21 but significantly increased at PND30 by Pb exposure. VDR expression in the brain was increased at both PND21 and PND30 (p < 0.05). These results suggest that Pb interferes with vitamin D metabolism by affecting the expression of its metabolizing enzymes. View Full-Text
Keywords: lead; vitamin D; 25-hydroxy vitamin D; 1,25-dihydroxy vitamin D; 25-hydroxylase; 1-α-hydroxylase; vitamin D receptor; rats lead; vitamin D; 25-hydroxy vitamin D; 1,25-dihydroxy vitamin D; 25-hydroxylase; 1-α-hydroxylase; vitamin D receptor; rats
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Rahman, A.; Al-Awadi, A.A.; Khan, K.M. Lead Affects Vitamin D Metabolism in Rats. Nutrients 2018, 10, 264.

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