Next Article in Journal
Impact of Genetic Variants on the Individual Potential for Body Fat Loss
Previous Article in Journal
In Vivo Protective Effects of Nootkatone against Particles-Induced Lung Injury Caused by Diesel Exhaust Is Mediated via the NF-κB Pathway
Open AccessArticle

Lead Affects Vitamin D Metabolism in Rats

Department of Food Science and Nutrition, College of Life Sciences, Kuwait University, P.O. Box 5969, Safat 13060, Kuwait
Department of Anatomy, Faculty of Medicine, Kuwait University, P.O. Box 24923, Safat 13110, Kuwait
Author to whom correspondence should be addressed.
Nutrients 2018, 10(3), 264;
Received: 4 January 2018 / Revised: 19 February 2018 / Accepted: 22 February 2018 / Published: 26 February 2018
A negative association between blood lead and vitamin D concentrations has been reported, however, experimental data on the effect of lead (Pb) on vitamin D metabolism is scarce. We investigated the effects of Pb on serum vitamin D metabolites, vitamin D activating enzymes and vitamin D receptor (VDR) in rats. Newborn Wistar rat pups were exposed to 0.2% Pb-acetate via their dams’ drinking water from post-natal day (PND) 1 to 21 and directly in drinking water until PND30. Serum 25-hydroxyvitamin D was analyzed with LC-MS/MS and 1,25-dihydroxyvitamin D with an immunoassay. Tissue expression of vitamin D activating enzymes and VDR were measured by Western blot and immunohistochemistry. Serum 25-hydroxyvitamin D was significantly decreased at both PND21 and PND30, whereas 1,25-dihydroxyvitamin D was decreased (p < 0.05) only at PND21 in the Pb-exposed rats. Expression of renal 1-α-hydroxylase was decreased by Pb only at PND21 (p < 0.05) but the brain 1-α-hydroxylase was not affected. Hepatic 25-hydroxylase expression was significantly decreased at PND21 but significantly increased at PND30 by Pb exposure. VDR expression in the brain was increased at both PND21 and PND30 (p < 0.05). These results suggest that Pb interferes with vitamin D metabolism by affecting the expression of its metabolizing enzymes. View Full-Text
Keywords: lead; vitamin D; 25-hydroxy vitamin D; 1,25-dihydroxy vitamin D; 25-hydroxylase; 1-α-hydroxylase; vitamin D receptor; rats lead; vitamin D; 25-hydroxy vitamin D; 1,25-dihydroxy vitamin D; 25-hydroxylase; 1-α-hydroxylase; vitamin D receptor; rats
Show Figures

Figure 1

MDPI and ACS Style

Rahman, A.; Al-Awadi, A.A.; Khan, K.M. Lead Affects Vitamin D Metabolism in Rats. Nutrients 2018, 10, 264.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop