Next Article in Journal
Mindfulness Is Associated with the Metabolic Syndrome among Individuals with a Depressive Symptomatology
Previous Article in Journal
Inadequate Iodine Intake in Population Groups Defined by Age, Life Stage and Vegetarian Dietary Practice in a Norwegian Convenience Sample
Open AccessArticle

Impact of Western and Mediterranean Diets and Vitamin D on Muscle Fibers of Sedentary Rats

1
Department of Clinical and Experimental Medicine, University of Catania, Via S. Sofia, 78, 95123 Catania, Italy
2
Department of Biomedical and Biotechnological Sciences, Human Anatomy and Histology Section, School of Medicine, University of Catania, Via S. Sofia, 87, 95123 Catania, Italy
*
Author to whom correspondence should be addressed.
Nutrients 2018, 10(2), 231; https://doi.org/10.3390/nu10020231
Received: 1 December 2017 / Revised: 28 January 2018 / Accepted: 14 February 2018 / Published: 17 February 2018
Background: The metabolic syndrome is associated with sarcopenia. Decreased serum levels of Vitamin D (VitD) and insulin-like growth factor (IGF)-1 and their mutual relationship were also reported. We aimed to evaluate whether different dietary profiles, containing or not VitD, may exert different effects on muscle molecular morphology. Methods: Twenty-eight male rats were fed for 10 weeks in order to detect early defects induced by different dietary regimens: regular diet (R); regular diet with vitamin D supplementation (R-DS) and regular diet with vitamin D restriction (R-DR); high-fat butter-based diets (HFB-DS and HFB-DR) with 41% energy from fat; high-fat extra-virgin olive oil-based diets (HFEVO-DS and HFEVO-DR) with 41% energy from fat. IL-1β, insulin-like growth factor (IGF)1, Dickkopf-1 (DKK-1), and VitD-receptor (VDR) expressions were evaluated by immunohistochemistry. Muscle fiber perimeter was measured by histology and morphometric analysis. Results: The muscle fibers of the HEVO-DS rats were hypertrophic, comparable to those of the R-DS rats. An inverse correlation existed between the dietary fat content and the perimeter of the muscle fibers (p < 0.01). In the HFB-DR rats, the muscle fibers appeared hypotrophic with an increase of IL-1β and a dramatic decrease of IGF-1 expression. Conclusions: High-fat western diet could impair muscle metabolism and lay the ground for subsequent muscle damage. VitD associated with a Mediterranean diet showed trophic action on the muscle fibers. View Full-Text
Keywords: Mediterranean diet; high-fat diet; skeletal muscle; vitamin D; extra-virgin olive oil Mediterranean diet; high-fat diet; skeletal muscle; vitamin D; extra-virgin olive oil
Show Figures

Figure 1

MDPI and ACS Style

Trovato, F.M.; Castrogiovanni, P.; Szychlinska, M.A.; Purrello, F.; Musumeci, G. Impact of Western and Mediterranean Diets and Vitamin D on Muscle Fibers of Sedentary Rats. Nutrients 2018, 10, 231.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop