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Open AccessArticle

High Fat/High Glucose Diet Induces Metabolic Syndrome in an Experimental Rat Model

Bioactividady Análisis de los Alimentos, Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM), Madrid 28049, Spain
Unidad Asociada I+D+i del Instituto de Investigación en Ciencias de la Alimentación (CIAL), Consejo Superior de Investigaciones Científicas (CSIC), Madrid 28049, Spain
Grupo de investigación en Biotecnología Alimentaria, Instituto de Investigaciones Biosanitarias, Universidad Francisco de Vitoria, Pozuelo de Alarcón, Madrid 28233, Spain
Departamento de Ciencias Básicas. Facultad de Ciencias de la Salud. Universidad Rey Juan Carlos, Alcorcón, Madrid 28922, Spain
NORT, Aix-Marseille Université, INRA, INSERM, Marseille 13385, France
Author to whom correspondence should be addressed.
Nutrients 2018, 10(10), 1502;
Received: 7 September 2018 / Revised: 9 October 2018 / Accepted: 12 October 2018 / Published: 14 October 2018
(This article belongs to the Special Issue Fructose and Glucose for Human Health)
PDF [2241 KB, uploaded 14 October 2018]


Metabolic syndrome (MetS) is defined as a constellation of many metabolic disorders such as hypertension, impaired glucose tolerance, dyslipidemia and obesity, being this last disorder a key factor in the etiology of the syndrome. The widespread of MetS in actual society, mainly in developed countries, is becoming an important health problem and is increasing the need to develop new treatments against this pathology is increasing fast. The main objective of the present study was to evaluate the MetS-associated alterations developed in a new glucose diet-induced-obesity (DIO) rodent model. These alterations were also compared to those alterations developed in a fructose-DIO rodent model. Wistar rats were divided into four groups: Control (C), High-fat (HF), High-fat/high-fructose (HFF) and High-fat/high-glucose (HFG). The animals were fed ad libitum for 20 weeks. At the end of the study, HFG animals showed lower expression of energy expenditure genes when compared to the other DIO groups. Oxidative stress biomarkers such as MDA and mitochondrial RT-qPCR analyses showed an increase of oxidative damage together with mitochondrial dysfunction in HFG group. This group also showed increased insulin and glucose plasma levels, though HFF animals showed the greatest increase on these parameters. All DIO groups showed increased plasma levels of triglycerides. Altogether, our results indicated a better impact of glucose than fructose, when combined with a high-fat diet, to induce most of the alterations associated with MetS in rats. In addition, our research facilitates a new animal model to evaluate future treatments for MetS. View Full-Text
Keywords: metabolic syndrome; obesity; diet induced obesity; rat model; high glucose; high fructose metabolic syndrome; obesity; diet induced obesity; rat model; high glucose; high fructose

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Moreno-Fernández, S.; Garcés-Rimón, M.; Vera, G.; Astier, J.; Landrier, J.F.; Miguel, M. High Fat/High Glucose Diet Induces Metabolic Syndrome in an Experimental Rat Model. Nutrients 2018, 10, 1502.

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