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Nutrients 2018, 10(10), 1436;

Maternal Protein Restriction Alters the Renal Ptger1 DNA Methylation State in SHRSP Offspring

Health Nutrition, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 1138657, Japan
Research Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 1130032, Japan
Department of Biochemistry, Kyushu University Graduate School of Medical Sciences, Kyushu University, Fukuoka 8128582, Japan
Author to whom correspondence should be addressed.
Received: 6 September 2018 / Revised: 25 September 2018 / Accepted: 28 September 2018 / Published: 5 October 2018
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We previously reported that maternal protein restriction (LP) during pregnancy increases salt sensitivity in offspring using the Stroke-Prone Spontaneously Hypertensive Rat (SHRSP). In the present study, we focus on DNA methylation profiles of prostaglandin E receptor 1 gene (ptger1), which is known to be associated with hypertension. We evaluated the ptger1 DNA methylation status via bisulfite sequencing, and analyzed the expression of ptger1-related genes. The results of these analyses showed that, compared to controls, the LP-S offspring exhibited both marked ptger1 hypermethylation, and significantly increased ptger1 expression. Moreover, they also exhibited significantly decreased expression of the downstream gene epithelial Na+ channel alpha (enacα). Interestingly, LP offspring that were provided with a standard water drinking supply (W) also exhibited increased ptger1 methylation and expression. Together, these results suggest that maternal protein restriction during pregnancy modulates the renal ptger1 DNA methylation state in SHRSP offspring, and thereby likely mediates ptger1 and enacα gene expression to induce salt sensitivity. View Full-Text
Keywords: maternal protein restriction; salt sensitivity; DNA methylation maternal protein restriction; salt sensitivity; DNA methylation

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Miyoshi, M.; Sato, M.; Saito, K.; Otani, L.; Shirahige, K.; Miura, F.; Ito, T.; Jia, H.; Kato, H. Maternal Protein Restriction Alters the Renal Ptger1 DNA Methylation State in SHRSP Offspring. Nutrients 2018, 10, 1436.

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