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Nutrients 2018, 10(1), 12;

Vitamin D Vitamers Affect Vitamin D Status Differently in Young Healthy Males

National Food Institute, Technical University of Denmark, 2800 Lyngby, Denmark
Institute of Mathematics and Computer Science, Technical University of Denmark, 2800 Lyngby, Denmark
Department of Nutrition, Exercise and Sports, University of Copenhagen, 1958 Frederiksberg C, Denmark
Author to whom correspondence should be addressed.
Received: 12 October 2017 / Revised: 18 December 2017 / Accepted: 19 December 2017 / Published: 23 December 2017
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Dietary intake of vitamin D includes vitamin D3 (vitD3), 25-hydroxyvitamin D3 (25OH-D3), and vitamin D2 (vitD2). However, the bioactivity of the different species has not been scientifically established. The hypothesis in this study was that vitD3, 25OH-D3, and vitD2 have an equal effect on 25-hydroxyvitamin D in serum (vitamin D status). To test our hypothesis, we performed a randomized, crossover study. Twelve young males consumed 10 µg/day vitD3 during a four-week run-in period, followed by 3 × 6 weeks of 10 µg/day vitD3, 10 µg/day 25OH-D3, and 10 µg/day vitD2. The content of vitD3, vitD2, 25OH-D3, and 25-hydroxyvitamin D2 (25OH-D2) in serum was quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The hypothesis that the three sources of vitamin D affect vitamin D status equally was rejected. Based on the assumption that 1 µg vitD3/day will show an increase in vitamin D status of 1.96 nmol/L, the results showed that 23 µg vitD2 and 6.8 µg 25OH-D3 was similar to 10 µg vitD3. These results demonstrate that further investigations are necessary to determine how to quantify the total vitamin D activity based on chemical quantification of the individual vitamin D metabolites to replace the total vitamin D activity assessed in biological rat models. View Full-Text
Keywords: 25-hydroxyvitamin D3; vitamin D2; vitamin D3; humans; bioactivity; supplements 25-hydroxyvitamin D3; vitamin D2; vitamin D3; humans; bioactivity; supplements
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Jakobsen, J.; Andersen, E.A.W.; Christensen, T.; Andersen, R.; Bügel, S. Vitamin D Vitamers Affect Vitamin D Status Differently in Young Healthy Males. Nutrients 2018, 10, 12.

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