Bioactivities and Chemical Compositions of Cinnamomum burmannii Bark Extracts (Lauraceae)
Round 1
Reviewer 1 Report
the manuscript is quite interesting for readers, however several comments are addressed to the authors in order to improve the quality of manuscript
1. line 12-please change "due to" to including
2. "This study aims to determine the antibacterial against Streptococcus 14 mutans and StaphylococcuS. aureus, antifungal against Candida albicans and Candida tropi- 15 calis, antioxidant and flavonoid content of Microwave Assisted Extraction (MAE) extracts 16 from C. burmanni bark" paraphrase to The objective of this study to determine the antibacterial against Streptococcus 14 mutans and StaphylococcuS. aureus, antifungal against Candida albicans and Candida tropi- 15 calis, antioxidant and flavonoid content of Microwave Assisted Extraction (MAE) extracts 16 from C. burmanni bark"
3. please stated the Abbreviation of DPPH and DMSO in the abstract
4. line 67-Microwave Assisted Extraction to microwave assisted extraction
5. line 69- Xiangang et al [number]? please revised all the manuscript
6. line 114 and 115- Indonesian 114 Institute of Sciences, Botanical Gardens Plant Conservation Center (city??)
7. please separated this subsection Plant Material, Chemicals and Reagents to the three part
8. please make the subsection MAE to the method
9. line 232 Antioxidantl Test?? typo
Author Response
RESPONSE TO REVIEWER 1 COMMENTS
- line 12-please change "due to" to including
Response: Thanks for the suggestion, we already changed from “due to” to “including”
- This study aims to determine the antibacterial against Streptococcus 14 mutans and StaphylococcuS. aureus, antifungal against Candida albicans and Candida tropi- 15 calis, antioxidant and flavonoid content of Microwave Assisted Extraction (MAE) extracts 16 from C. burmanni bark" paraphrase to The objective of this study to determine the antibacterial against Streptococcus 14 mutans and StaphylococcuS. aureus, antifungal against Candida albicans and Candida tropi- 15 calis, antioxidant and flavonoid content of Microwave Assisted Extraction (MAE) extracts 16 from C. burmanni bark"
Response: Thanks for the suggestion. We already changed to “The objective of this study to determine the antibacterial against Streptococcus mutans and Staphylococcus. aureus, antifungal against Candida albicans and Candida tropicalis, antioxidant and flavonoid content of Microwave Assisted Extraction (MAE) extracts 16 from C. burmanni bark"
- please stated the Abbreviation of DPPH and DMSO in the abstract
Response: We already stated the Abbreviation of DPPH and DMSO in the abstract. The 1,1-diphenyl-2-pikrilhidrazil (DPPH) method was used for the antioxidant test, the agar dilution method for the Minimum Inhibitory Concentration (MIC), and the paper disc diffusion method for the Width of the Inhibitory Area (LDH). The positive antifungal control was nystatin, while the antibacterial control was amoxicillin, and both negative controls were 1% dimethyl sulfoxide (DMSO).
- line 67-Microwave Assisted Extraction to microwave assisted extraction
Response: we already changed to The method currently being developed is microwave assisted extraction (MAE)
- line 69- Xiangang et al [number]? please revised all the manuscript
Response: Thanks for the correction, Xiangang reference number is [19] whose name is Xiangang Hu, and we have changed “Hu et. al. stated that the extraction activity of antibacterial is better than the maceration method [19,20]”
- line 114 and 115- Indonesian Institute of Sciences, Botanical Gardens Plant Conservation Center (city??)
Response:We already added Bogor as the city of Indonesian Institute of Sciences, Botanical Gardens Plant Conservation Center
- please separated this subsection Plant Material, Chemicals and Reagents to the three part
Response: We already separated to three parts and we already added the tool part
2.1. Plant Material, Chemicals and Reagents
The material used was dry C. burmanii bark obtained from the Research Institute for Spices and Medicinal Plants and determined at the Indonesian Institute of Sciences, Botanical Gardens Plant Conservation Center, Bogor. Others include isolates of S. mutans, S. aureus, C. albicans and C. tropicalis fungi from the Microbiology Laboratory, Bogor Agricultural University.
2.2. Chemicals and Reagents
The chemicals and reagents that were used are amoxicillin and nystatin as a positive control for antibacterial and antifungal testing from Kimia Farma brand. Furthermore, Dimethyl Sulfoxide 1% was used as a negative control, in addition to hydrochloric acid, 96% ethanol, iron (III) chloride solution, magnesium powder, Nutrient Agar medium, Potato Dextrose Agar medium, Bouchardat reagent, Dragendorf, Mayer, from German sigma. The solvents include n-hexane, ethyl acetate, ethanol, methanol, DPPH powder, quercetin, alumunium chloride, sodium acetate, and magnesium powder. The study was carried out in several stages, the first by making Simplicia powder. The second is by extracting the MAE method with three solvents, followed by the third stage, which qualitatively screens the extracts to determine the content of alkaloids, flavonoids, tannins, saponins, steroids, and terpenoids. The fourth stage is determining the extracted flavonoid content, while the fifth step is testing the antioxidant activity using the DPPH method. The sixth stage is the Minimum Inhibitory Concentration (MIC) test, and the seventh is the Inhibitory Area Width (IAW) Test.
2.3. The Tools
The tools which were used include microwave (Samsung®), digital balance (Lab Pro®), analytical balance (And®), UV-Vis spectrophotometer (Jasco V-730®), the crucible, thermometer, furnace (Daihan Scientific Furnace®), Oven (Memmert®), Mesh 40, Filter Paper, Aluminum Foil And Glassware (Pyrex®), autoclave (All American®), bunsenes, steam plate, Petri dish (Pyrex®), grinder (Airlux®), incubator (Memmert®), paper disc (Whatman number 40) diameter 6 mm, micipipette (Proline plus®), oven (Memmert®), 40 mesh sieve, and furnace (Vulcan A-550®).
- please make the subsection MAE to the method
Response:The subsection MAE has been added to
2.5. Extracts Preparations using MAE Method
- line 232 Antioxidantl Test?? Typo
Response. Thankyou, yes it was typo and we already fixed.
Reviewer 2 Report
Some suggestions to improve the text.
Comments for author File: 
Comments.pdf
Author Response
RESPONSE TO REVIEWER 2 COMMENTS
- Page 3, line 143, changed from 500C to 500oC
Response: Thanks for the suggestion, we already changed
- Page 5, line 224, changed from 10mL to 10 mL and y=bx=+a to Y = bx + a
Response: Thanks for the suggestion. We already changed
- Page 6: line 254, changed from 23g to 23 g; line 265 changed from Farland. [42–44].to Farland [42–44]; line 281 changed from 20mL to 20 mL
Response: we already changed as you suggested
- Page 9; line 380 changed from standard victim C. to standard vitamin C and line 392 changed from 313.628 ppm, 163,010 ppm, and 76,490 ppm. To 313.628 ppm, 163.010 ppm, and 76.490 ppm.
Response: We already rechecked and changed
- Page 13; line 518, changed from (0-20mm) to (0-20 mm), and line 519, changed from (5-10mm) to (5-10 mm)
Response:We already changed as your comment
- In tables 1, 3, 4, 5, 6, 7 and 8 the decimal places are separated by commas when they should be separated by dots. In several measurements in the tables, the number of decimal places of the errors is higher than the measurement itself. The error is related to the number of decimal places of the measuring instrument used!!
Response:We already revised all of the commas and all of the numbers have been separated by dots.
Reviewer 3 Report
dear auhtors please make all changes in the attached PDF file.
Comments for author File: Comments.pdf
Author Response
RESPONSE TO REVIEWER 3 COMMENTS
- line 13, changed from “essential oil of cinnamaldehyde, eugenol, and safrole” to “essential oil content of cinnamaldehyde, eugenol, and safrole.”
Response: Thanks for the suggestion, we already changed
- Line 34, changed from cinnamaldehyde essential oil to “cinnamaldehyde in the essential oil”
Response: Thanks for the suggestion. We already changed
- “of” delete it from sentence burmanii contained of cinnamaldehyde compounds
Response: We already deleted
- Line 45, added the word “against”
Response: We already checked and added “stated that the secondary metabolites used as antimicrobial and antifungal against B. cereus, S. aureus, E. coli, P. aeruginosa, C. Albicans, S. mutans, and Klebsiella sp are cinnamaldehyde and cinnamic acid [6,7].”
- Line 69-70 please changed to Xiangang et. Al. stated that antibacterial activity of MAE is better than the maceration method
Response: We already revised the sentence as reviewer wants and we changed the name of the reference to be Hu. Because the name of the first author is Xiangang Hu.
- Line 74 change “with” to “and”.
Response: We already revised, thanks.
- Line 143, 500C change to 500 oC
Response: We already added the degree symbol
- Line 249, 1210C change to 1210 oC
Response: We already added the degree symbol
- Line 380 recheck the word
Response: We already corrected the word to be Vitamin C
- Line 496 Wiyanto (2010) change to reference style
Response: We already changed to “Wiyanto [55]”
- The reference number [46] added the year of publication
Response: We already added the year.
