Environmental Fate of Multistressors on Carpet Shell Clam Ruditapes decussatus: Carbon Nanoparticles and Temperature Variation
Abstract
:1. Introduction
2. Material and Methods
2.1. Contaminants
2.1.1. Description
2.1.2. Characterization
2.2. Sampling and Laboratory Conditions
2.3. Biologic Analyses
2.4. Data Analyses
2.4.1. Principal Coordinates Analysis
2.4.2. Statistical Analysis
3. Results and Discussion
3.1. Characterization Analysis
3.2. Biological Analyses
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Technical Data | ||||||
---|---|---|---|---|---|---|
MWCNTs–COOH | Diameter (nm) | Length (um) | Carbon Purity (%) | Surface Area (m2/g) | Amorphous Carbon (mol%) | -COOH (wt%) |
2–5 | 10–30 | 98 | 400 | 8–10 | 3.86 |
Temperature (°C) | |||||||||
---|---|---|---|---|---|---|---|---|---|
10 | 15 | 20 | 25 | ||||||
T0 | T96 | T0 | T96 | T0 | T96 | T0 | T96 | ||
MWCNTs–COOH | Particle size (nm) | 2307.1 | 2289.1 | 2564.3 | 2500.1 | 2601.2 | 2790.8 | 3989.1 | 4001.2 |
PDI | 0.98 | 0.97 | 1.01 | 0.91 | 1.13 | 1.16 | 0.26 | 0.33 |
Temperature (°C) | ||||||
---|---|---|---|---|---|---|
10 | 15 | 20 | 25 | |||
Energy reserves content | PROT (mg/g) | CTL | 80.99 ± 19.10 A | 93.68 ± 17.53 A | 91.23 ± 9.59 A | 89.12 ± 20.84 A |
Exp | 89.24 ± 9.92 a | 102.87 ± 15.47 a | 78.46 ± 15.08 a | 81.40 ± 21.01 a | ||
GLY (mg/g) | CTL | 1.37 ± 0.36 A | 1.63 ± 0.30 A | 1.95 ± 0.52 B | 1.58 ± 0.21 A | |
Exp | 1.65 ± 0.41 a | 1.60 ± 0.49 a | 1.42 ± 0.50 a | 1.65 ± 0.11 a | ||
Antioxidant defenses | CAT (U/g) | CTL | 9.25 ± 1.65 A | 10.83 ± 1.70 A | 9.92 ± 2.23 A | 11.10 ± 1.98 A |
Exp | 8.87 ± 0.87 a | 10.58 ± 0.49 a | 10.28 ± 1.50 a | 9.93 ± 0.79 a | ||
GPx (U/g) | CTL | 0.05 ± 0.02 A | 0.05 ± 0.02 A* | 0.10 ± 0.02 A | 0.06 ± 0.02 A | |
Exp | 0.08 ± 0.02 a | 0.13 ± 0.01 b | 0.14 ± 0.01 b | 0.14 ± 0.02 b | ||
Neuro status | AChE (nmol/min/g) | CTL | 0.08 ± 0.02 A | 0.09 ± 0.01 A | 0.08 ± 0.02 A | 0.18 ± 0.05 B,* |
Exp | 0.09 ± 0.02 a | 0.07 ± 0.01 a | 0.06 ± 0.01 a | 0.01 ± 0.00 b |
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De Marchi, L.; Rocha, R.J.M.; Rodrigues, A.C.M.; Soares, A.M.V.M.; Pretti, C.; Chiellini, F.; Freitas, R. Environmental Fate of Multistressors on Carpet Shell Clam Ruditapes decussatus: Carbon Nanoparticles and Temperature Variation. Sustainability 2020, 12, 4939. https://doi.org/10.3390/su12124939
De Marchi L, Rocha RJM, Rodrigues ACM, Soares AMVM, Pretti C, Chiellini F, Freitas R. Environmental Fate of Multistressors on Carpet Shell Clam Ruditapes decussatus: Carbon Nanoparticles and Temperature Variation. Sustainability. 2020; 12(12):4939. https://doi.org/10.3390/su12124939
Chicago/Turabian StyleDe Marchi, Lucia, Rui Jorge Miranda Rocha, Andreia C.M. Rodrigues, Amadeu M.V.M. Soares, Carlo Pretti, Federica Chiellini, and Rosa Freitas. 2020. "Environmental Fate of Multistressors on Carpet Shell Clam Ruditapes decussatus: Carbon Nanoparticles and Temperature Variation" Sustainability 12, no. 12: 4939. https://doi.org/10.3390/su12124939