Comparative Study of Δ9-Tetrahydrocannabinol and Cannabidiol on Melanogenesis in Human Epidermal Melanocytes from Different Pigmentation Phototypes: A Pilot Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Cell Culture
2.3. Cytotoxicity Assay
2.4. Intracellular Melanin Assay
2.5. Intracellular Tyrosinase Activity
2.6. Measurement of Melanocyte Dendricity
2.7. Recovery Study of Dendricity
2.8. Intracellular Reactive Oxygen Species (ROS)
2.9. Statistical Analysis
3. Results
3.1. Effects of Compounds on Viability of HEMn-LP and HEMn-DP Cells
3.2. Effects of Compounds on Melanin Synthesis, Tyrosinase Activity and Melanocyte Dendricity in HEMn-LP Cells
3.3. Effects of Compounds on Melanin Synthesis, Tyrosinase Activity and Melanocyte Dendricity in HEMn-DP Cells
3.4. Effects of Compounds on Recovery of Melanocyte Dendricity in HEMn-LP Cells
3.5. Effects of Compounds on Intracellular ROS Generation in HEMn-LP and HEMn-DP Cells
4. Discussion
Supplementary Materials
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Goenka, S. Comparative Study of Δ9-Tetrahydrocannabinol and Cannabidiol on Melanogenesis in Human Epidermal Melanocytes from Different Pigmentation Phototypes: A Pilot Study. J. Xenobiot. 2022, 12, 131-144. https://doi.org/10.3390/jox12020012
Goenka S. Comparative Study of Δ9-Tetrahydrocannabinol and Cannabidiol on Melanogenesis in Human Epidermal Melanocytes from Different Pigmentation Phototypes: A Pilot Study. Journal of Xenobiotics. 2022; 12(2):131-144. https://doi.org/10.3390/jox12020012
Chicago/Turabian StyleGoenka, Shilpi. 2022. "Comparative Study of Δ9-Tetrahydrocannabinol and Cannabidiol on Melanogenesis in Human Epidermal Melanocytes from Different Pigmentation Phototypes: A Pilot Study" Journal of Xenobiotics 12, no. 2: 131-144. https://doi.org/10.3390/jox12020012