Next Article in Journal
Effect of Catanospermine, 1-Deoxynojirimycin or 1-Deoxymannojirimycin on Biological and Functional Activities of Japanese Encephalitis Virus in Porcine Stable Kidney Cells
Previous Article in Journal
Lasiodiplodia theobromae Keratitis: A Rare Fungi from Eastern India
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Microbiology Research
Volume 4
Issue 1
10.4081/mr.2013.e1
microbiolres-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
Microbiology Research is published by MDPI from Volume 11 Issue 2 (2020). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with PAGEPress.
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Molecular Epidemiology of GB Type C Virus among Individuals Exposed to Hepatitis C Virus in Cameroon

by
Judith N. Torimiro
1,2,*,
Qing Mao
3,
Nathan D. Wolfe
4,
Ubald Tamoufe
3,
Ana Weil
5,
Eitel Mpoudi Ngole
6,
Donald S. Burke
6,
Stuart C. Ray
3 and
Dale Netski
3
1
Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon
2
Chantal Biya International Reference Centre (CIRCB), Yaounde, Cameroon
3
Johns Hopkins School of Medicine, Baltimore, MD, USA
4
Global Virus Forecasting Initiative, Cameroon
5
Army Health Research Centre (CREMER),Yaounde, Cameroon
6
University of Pittsburgh, Pittsburgh, PA, USA
*
Author to whom correspondence should be addressed.
Microbiol. Res. 2013, 4(1), e1; https://doi.org/10.4081/mr.2013.e1
Submission received: 1 October 2012 / Revised: 28 May 2012 / Accepted: 11 June 2012 / Published: 2 April 2013

Abstract

GB Virus Type C (GBV-C), a blood-borne flavivirus currently infects about one sixth of the world’s population. Its transmission has been reported through parenteral, sexual and vertical routes. Unusually for RNA viruses, it exhibits a high degree of conservation of the polyprotein sequence. The geographical distribution of GBV-C suggests an African origin and a long-term co-evolution in the human population but without any known pathogenicity. The aim of this study was to describe the different sub-types of this virus in Southern Cameroon. We studied the genetic epidemiology of GBV-C among rural populations where many HIV-1 and HCV genotypes have been identified. Plasma samples of 345 subjects with evidence of HCV exposure were tested for GBV-C infection. To detect GBV-C RNA, reverse transcription followed by a nested PCR of 5’UTR were performed. Direct sequencing and phylogenetic studies using PHYLIP, PAUP* and SimPlot were carried out. In total, 31 GBV-C RNA-positive samples were detected giving a prevalence of 9.0% among HCV-exposed individuals. Phylogenetic analysis of the 5’UTR showed two distinct clusters: Genotype 1 and Genotype 2. Twenty-eight isolates (8.0%) clustered with Genotype 1 and 3 (1.0%) with Genotype 2. More than one genotype of GBV-C is prevalent in Cameroon of which GBV-C Genotype 1 is more common, confirming reports in the literature. Studying the near full-length genome sequences of GBV-C isolates from primates in this region may provide clues of viral recombination, evolution and origin.
Keywords: GBV-C; flavivirus; Cameroon; phylogeny; genotype GBV-C; flavivirus; Cameroon; phylogeny; genotype

Share and Cite

MDPI and ACS Style

Torimiro, J.N.; Mao, Q.; Wolfe, N.D.; Tamoufe, U.; Weil, A.; Mpoudi Ngole, E.; Burke, D.S.; Ray, S.C.; Netski, D. Molecular Epidemiology of GB Type C Virus among Individuals Exposed to Hepatitis C Virus in Cameroon. Microbiol. Res. 2013, 4, e1. https://doi.org/10.4081/mr.2013.e1

AMA Style

Torimiro JN, Mao Q, Wolfe ND, Tamoufe U, Weil A, Mpoudi Ngole E, Burke DS, Ray SC, Netski D. Molecular Epidemiology of GB Type C Virus among Individuals Exposed to Hepatitis C Virus in Cameroon. Microbiology Research. 2013; 4(1):e1. https://doi.org/10.4081/mr.2013.e1

Chicago/Turabian Style

Torimiro, Judith N., Qing Mao, Nathan D. Wolfe, Ubald Tamoufe, Ana Weil, Eitel Mpoudi Ngole, Donald S. Burke, Stuart C. Ray, and Dale Netski. 2013. "Molecular Epidemiology of GB Type C Virus among Individuals Exposed to Hepatitis C Virus in Cameroon" Microbiology Research 4, no. 1: e1. https://doi.org/10.4081/mr.2013.e1

APA Style

Torimiro, J. N., Mao, Q., Wolfe, N. D., Tamoufe, U., Weil, A., Mpoudi Ngole, E., Burke, D. S., Ray, S. C., & Netski, D. (2013). Molecular Epidemiology of GB Type C Virus among Individuals Exposed to Hepatitis C Virus in Cameroon. Microbiology Research, 4(1), e1. https://doi.org/10.4081/mr.2013.e1

Article Metrics

Back to TopTop