Effect of Buffer Room Configuration on Isolation of Bacteriophage phi6 and Micrococcus Luteus Emissions

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

#Abstarct very less information. rewrite it again. 

#Introduction 

Line 27,29 why you use so many reference ? do you read all of this paper ? if not please reduce and give perfect one.

line 34 where is your reference ?

Buffer room, what is your evidence ? why you choose this ?

#Materials and Methods

there is no section. We cannot understand. Please divided into section.

there is only some section for example test apparatus.

Figure 8 is not clear. Make it 300 dpi.

#Discussion is not enough. Please rewrite it. 

 

 

Comments on the Quality of English Language

ok

Author Response

Thank you very much for taking the time to review this manuscript and for your valuable comments, which in the future will allow more accurate rephrasing of the research results. All comments have been corrected in the text of the article. Below are the answers to each question.

C 1#Abstarct very less information. rewrite it again. 

The abstract has been enhanced by including specific data on the global impact of the SARS-CoV-2 virus, which has led to over 770 million infections and 7 million deaths worldwide. This addition highlights the critical importance of the study, which investigates how different buffer room configurations affect microbial isolation. The new information underscores the urgency of developing effective architectural solutions for medical and public spaces, particularly during pandemics. These enhancements provide a stronger context and justification for the research, emphasizing its relevance in the ongoing battle against viral transmission.

#Introduction 

C2 Line 27,29 why you use so many reference ? do you read all of this paper ? if not please reduce and give perfect one.

Each reference cited in lines 27 and 29 has been carefully reviewed and deemed essential by the authors. In summary, all the referenced papers were read and are considered crucial for a comprehensive and well-rounded understanding of the subject matter. Reducing the number of references would undermine the depth and breadth of the literature review and analysis presented in the study.

C3 line 34 where is your reference ?

The article is quoted below

C4 Buffer room, what is your evidence ? why you choose this ?

#Materials and Methods

C5 there is no section. We cannot understand. Please divided into section.

The chapter has been divided into additional sections

C6 there is only some section for example test apparatus.

This section describes the key research equipment that was used during the experiment

C7 Figure 8 is not clear. Make it 300 dpi.

Photo no. 8 has been changed to a higher resolution

C8 #Discussion is not enough. Please rewrite it. 

The discussion has been completed

Reviewer 2 Report

Comments and Suggestions for Authors

The manuscript by Radosław Wróbel and colleagues reports the results of studies of influence of the configuration of buffer rooms on the degree of isolation of a source of emission of phages and bacteria.

 

The authors conducted careful experimental work and offered a scrupulous analysis of comparisons of effectiveness of different versions of the infective room isolation system. The results of this study can be used in the design of hospitals and laboratories, which makes this research relevant. The design of experiments is logical and straightforward, the test procedure looks reliable, the data are presented convincingly, the illustrations contribute understanding, and the discussion part is comprehensive. Overall, the paper can be considered for publication in the journal Microbiology Research.

 

However, the manuscript lacks an explanation as to why they chose Micrococcus luteus ATCC 7468 and Phi 6. Besides, they could explain how the stability of phages could influence the results of experiments.

Author Response

Thank you very much for taking the time to review this manuscript and for your valuable comments, which in the future will allow more accurate rephrasing of the research results. All comments have been corrected in the text of the article. Below are the answers to a question.

 

 

The authors conducted careful experimental work and offered a scrupulous analysis of comparisons of effectiveness of different versions of the infective room isolation system. The results of this study can be used in the design of hospitals and laboratories, which makes this research relevant. The design of experiments is logical and straightforward, the test procedure looks reliable, the data are presented convincingly, the illustrations contribute understanding, and the discussion part is comprehensive. Overall, the paper can be considered for publication in the journal Microbiology Research.

 

C1. However, the manuscript lacks an explanation as to why they chose Micrococcus luteus ATCC 7468 and Phi 6. Besides, they could explain how the stability of phages could influence the results of experiments.

 

The microorganisms were selected on the basis of previous research to which there is a bibliographic reference in the text (Wróbel R, Andrych-Zalewska M, Matla J, Molska J, Sierzputowski G, Szulak A, et al. Assessment of the Possibility of Using Bacterial Strains and Bacteriophages for Epidemiological Studies in the Bioaerosol Environment. Microbiol Res (Pavia) [Internet]. 2024 Feb 6;15(1):236–46. Available from: https://www.mdpi.com/2036-7481/15/1/17.).

Two kinds of bioaerosol – one from the group of bacteria and one from the group of bacteriophages (viruses) – were selected for effectiveness testing. On the basis of experiments Micrococcus luteus ATCC 7468 was selected from the group of bacteria. Microbiological examinations showed that in the case of this microorganism a countable bioaerosol could be obtained from a suspension with turbidity within the McFarland scale, a bacterial colony (its colour, shape and size) could be easily identified, and that this bacterium could survive in a physiological solution, artificial saliva and air. On the basis of experiments phage phi6 was selected from the group of bacteriophages. Microbiological examinations showed that in the case of this microorganism a countable bioaerosol could be obtained and viral plaques (clear areas) could be easily identified, and that this bacteriophage could survive in a physiological solution.

Round 2

Reviewer 1 Report

Comments and Suggestions for Authors

Ok

Comments on the Quality of English Language

Moderate