Clonality Analysis of Streptococcus pneumoniae in Clinical Specimens
Abstract
:1. Introduction
2. Materials and Methods
2.1. Samples
2.2. Sample Pretreatment and Nucleic Acid Extraction
2.3. Real-Time PCR
2.4. Clonality Analysis by Sanger Sequencing
2.4.1. Control Strains
2.4.2. PCR of cpsB Gene
2.4.3. Nucleotide Sequencing
2.4.4. Clonality Identification
2.5. Statistical Analysis
3. Results
3.1. Detection of Pneumococcus from Clinical Specimens Using Real-Time PCR
3.2. Clonality Analysis Using Sanger Sequencing
3.3. Clonality Tendencies by Underlying Factors
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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No | Sample Code | Real-Time PCR (Ct) | Sanger Sequencing Results * | |
---|---|---|---|---|
Clone(s) Number | Interpretation ** | |||
1 | C5136 | 11.9 | 1 | Single |
2 | A5001 | 12.08 | 5 | Multiple |
3 | C5119 | 13.05 | 1 | Single |
4 | C5004 | 14.31 | 1 | Single |
5 | C5071 | 15.94 | 1 | Single |
6 | C5003 | 15.98 | 4 | Multiple |
7 | A5019 | 16.74 | 1 | Single |
8 | C5134 | 17.83 | 1 | Single |
9 | C5124 | 18.09 | 5 | Multiple |
10 | C5014 | 18.56 | 4 | Multiple |
11 | C5078 | 18.8 | 1 | Single |
12 | D5017 | 19.47 | 1 | Single |
13 | D5008 | 21.8 | 1 | Single |
14 | A5004 | 23.53 | 3 | Multiple |
15 | C5133 | 24.44 | 4 | Multiple |
16 | C5108 | 24.67 | 4 | Multiple |
17 | C5069 | 26.79 | 4 | Multiple |
18 | C5109 | 28.8 | 4 | Multiple |
19 | D5007 | 29.8 | 6 | Multiple |
20 | A5014 | 30.17 | 4 | Multiple |
21 | C5019 | 30.38 | 4 | Multiple |
22 | C5075 | 30.38 | 3 | Multiple |
23 | C5095 | 31.16 | 3 | Multiple |
24 | C5005 | 31.65 | 4 | Multiple |
25 | A5018 | 32.0 | 1 | Single |
26 | C5017 | 32.17 | 3 | Multiple |
27 | C5110 | 32.22 | 4 | Multiple |
28 | C5107 | 32.71 | 1 | Single |
29 | C5074 | 33.01 | 4 | Multiple |
30 | C5024 | 33.21 | 4 | Multiple |
31 | A5016 | 33.92 | 3 | Multiple |
32 | C5104 | 34.17 | 4 | Multiple |
33 | C5137 | 34.7 | 5 | Multiple |
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Lestari, D.C.; Somboonthum, P.; Motooka, D.; Ishii, E.; Matsuda, S.; Karuniawati, A.; Iida, T. Clonality Analysis of Streptococcus pneumoniae in Clinical Specimens. Microbiol. Res. 2024, 15, 1110-1118. https://doi.org/10.3390/microbiolres15030074
Lestari DC, Somboonthum P, Motooka D, Ishii E, Matsuda S, Karuniawati A, Iida T. Clonality Analysis of Streptococcus pneumoniae in Clinical Specimens. Microbiology Research. 2024; 15(3):1110-1118. https://doi.org/10.3390/microbiolres15030074
Chicago/Turabian StyleLestari, Delly Chipta, Pranee Somboonthum, Daisuke Motooka, Eiji Ishii, Shigeaki Matsuda, Anis Karuniawati, and Tetsuya Iida. 2024. "Clonality Analysis of Streptococcus pneumoniae in Clinical Specimens" Microbiology Research 15, no. 3: 1110-1118. https://doi.org/10.3390/microbiolres15030074
APA StyleLestari, D. C., Somboonthum, P., Motooka, D., Ishii, E., Matsuda, S., Karuniawati, A., & Iida, T. (2024). Clonality Analysis of Streptococcus pneumoniae in Clinical Specimens. Microbiology Research, 15(3), 1110-1118. https://doi.org/10.3390/microbiolres15030074