Biocompatibility Assessment of Polycaprolactone/Polylactic Acid/Zinc Oxide Nanoparticle Composites under In Vivo Conditions for Biomedical Applications
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of ZnO-NPs
2.3. Preparation of PCL/PLA/ZnO-NP Blocks
2.4. Characterization of the ZnO-NPs
2.5. Characterization of PCL/PLA/ZnO-NP Blocks
2.5.1. Fourier Transform Infrared Spectroscopy and X-ray Diffraction
2.5.2. Thermal Analysis of the PCL/PLA/ZnO-NP Blocks
2.5.3. Microstructure Studies
2.6. In Vivo Biocompatibility Studies of the PCL/PLA/ZnO-NP Blocks
2.6.1. Surgical Preparation of Biomodels
2.6.2. Histological Analysis
3. Results and Discussion
3.1. Characterization of ZnO-NPs
3.2. FT-IR of PCL/PLA/ZnO-NPs Blocks
3.3. XRD of the PCL/PLA/ZnO-NP Blocks
3.4. Thermal Analysis of PCL/PLA/ZnO-NPs Blocks
3.5. Scanning Electron Microscopy (SEM) of PCL/PLA/ZnO-NP Blocks
3.6. In Vivo Biocompatibility Tests of the PCL/PLA/ZnO-NP Blocks
3.6.1. Biocompatibility of Formulation F1
3.6.2. Biocompatibility of Formulation F2
3.6.3. Biocompatibility of Formulation F3
3.6.4. Biocompatibility of Formulation F4
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Components | PCL (%) | PLA (%) | ZnO-NPs (%) |
---|---|---|---|
F1 | 30 | 70 | 0 |
F2 | 29.5 | 70 | 0.5 |
F3 | 29 | 70 | 1 |
F4 | 28 | 70 | 2 |
Tg (°C) | Tcc (°C) | Tm1 (°C) | Tm2 (°C) | Tm3 (°C) | Xc PLA (%) | |
---|---|---|---|---|---|---|
F1 | 55 | 84 | 128 | 143 | 52 | 5.1 |
F2 | 57 | 89 | 134 | 147 | 52 | 4.9 |
F3 | 55 | 84 | 134 | - | 52 | 4.7 |
F4 | 52 | - | - | 144 | 68 | - |
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Castro, J.I.; Araujo-Rodríguez, D.G.; Valencia-Llano, C.H.; López Tenorio, D.; Saavedra, M.; Zapata, P.A.; Grande-Tovar, C.D. Biocompatibility Assessment of Polycaprolactone/Polylactic Acid/Zinc Oxide Nanoparticle Composites under In Vivo Conditions for Biomedical Applications. Pharmaceutics 2023, 15, 2196. https://doi.org/10.3390/pharmaceutics15092196
Castro JI, Araujo-Rodríguez DG, Valencia-Llano CH, López Tenorio D, Saavedra M, Zapata PA, Grande-Tovar CD. Biocompatibility Assessment of Polycaprolactone/Polylactic Acid/Zinc Oxide Nanoparticle Composites under In Vivo Conditions for Biomedical Applications. Pharmaceutics. 2023; 15(9):2196. https://doi.org/10.3390/pharmaceutics15092196
Chicago/Turabian StyleCastro, Jorge Iván, Daniela G. Araujo-Rodríguez, Carlos Humberto Valencia-Llano, Diego López Tenorio, Marcela Saavedra, Paula A. Zapata, and Carlos David Grande-Tovar. 2023. "Biocompatibility Assessment of Polycaprolactone/Polylactic Acid/Zinc Oxide Nanoparticle Composites under In Vivo Conditions for Biomedical Applications" Pharmaceutics 15, no. 9: 2196. https://doi.org/10.3390/pharmaceutics15092196
APA StyleCastro, J. I., Araujo-Rodríguez, D. G., Valencia-Llano, C. H., López Tenorio, D., Saavedra, M., Zapata, P. A., & Grande-Tovar, C. D. (2023). Biocompatibility Assessment of Polycaprolactone/Polylactic Acid/Zinc Oxide Nanoparticle Composites under In Vivo Conditions for Biomedical Applications. Pharmaceutics, 15(9), 2196. https://doi.org/10.3390/pharmaceutics15092196