Antibacterial and Antifungal Properties of a Novel Antimicrobial Peptide GK-19 and Its Application in Skin and Soft Tissue Infections Induced by MRSA or Candida albicans
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Characterization of the Physicochemical Properties of the Antimicrobial Peptides
2.3. Antimicrobial Peptides Stability Assay
2.4. Bacteria and Fungi Strains Preparation and Growth Conditions
2.5. Antimicrobial Assay
2.6. Antimicrobial Mechanism
2.7. Cells and Animals
2.8. In Vitro Cytotoxicity Assay
2.9. In Vivo Cytotoxicity Assay
2.10. Hemolysis Assay
2.11. The Scalded Mice Models Combined with SSTIs
2.12. In Vivo Anti-SSTIs Assay
2.13. Histological Analysis
2.14. Statistical Analysis
3. Results
3.1. Peptide Functional Screening Showed a Prolonged Half-Life of GK-19
3.2. GK-19 Potently Kills a Broad Range of Both Bacteria and Fungi by Permeabilizing the Microbial Membrane
3.3. GK-19 Showed Negligible Toxicity Both In Vitro and In Vivo
3.4. GK-19 Promoted the Wound Healing of Mice against SSTIs Caused by MRSA
3.5. GK-19 Promoted the Wound Healing of Mice against SSTIs Caused by C. albicans
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Peptide | AamAP1 | AamAP1-Lysine | GK-19 |
---|---|---|---|
Sequence | FLFSLIPHAIGGLISAFK | FLFKLIPKAIKKLISKFK | GFLFKLIPKAIKKLISKFK |
Length | 18 | 18 | 19 |
Hydrophobicity (H) | 0.904 | 0.607 | 0.575 |
% Helicity | 72.22 | 88.3 | 94.74 |
Net Charge z | +1.1 | +6 | +6 |
Polar Residues (n/%) | 6/33.3 | 7/38.89 | 8/42.11 |
Nonpolar Residues (n/%) | 12/66.67 | 11/61.11 | 11/57.89 |
Water Solubility | poor | good | good |
Estimated Half-Life | 1.1 h (mammalian reticulocytes, in vitro); 3 min (yeast, in vivo); 2 min (Escherichia coli, in vivo) | 30 h; >20 h; >10 h |
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Song, C.; Wen, R.; Zhou, J.; Zeng, X.; Kou, Z.; Zhang, J.; Wang, T.; Chang, P.; Lv, Y.; Wu, R. Antibacterial and Antifungal Properties of a Novel Antimicrobial Peptide GK-19 and Its Application in Skin and Soft Tissue Infections Induced by MRSA or Candida albicans. Pharmaceutics 2022, 14, 1937. https://doi.org/10.3390/pharmaceutics14091937
Song C, Wen R, Zhou J, Zeng X, Kou Z, Zhang J, Wang T, Chang P, Lv Y, Wu R. Antibacterial and Antifungal Properties of a Novel Antimicrobial Peptide GK-19 and Its Application in Skin and Soft Tissue Infections Induced by MRSA or Candida albicans. Pharmaceutics. 2022; 14(9):1937. https://doi.org/10.3390/pharmaceutics14091937
Chicago/Turabian StyleSong, Chenghua, Ruichao Wen, Jiaxuan Zhou, Xiaoyan Zeng, Zi Kou, Jia Zhang, Tao Wang, Pengkang Chang, Yi Lv, and Rongqian Wu. 2022. "Antibacterial and Antifungal Properties of a Novel Antimicrobial Peptide GK-19 and Its Application in Skin and Soft Tissue Infections Induced by MRSA or Candida albicans" Pharmaceutics 14, no. 9: 1937. https://doi.org/10.3390/pharmaceutics14091937
APA StyleSong, C., Wen, R., Zhou, J., Zeng, X., Kou, Z., Zhang, J., Wang, T., Chang, P., Lv, Y., & Wu, R. (2022). Antibacterial and Antifungal Properties of a Novel Antimicrobial Peptide GK-19 and Its Application in Skin and Soft Tissue Infections Induced by MRSA or Candida albicans. Pharmaceutics, 14(9), 1937. https://doi.org/10.3390/pharmaceutics14091937