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Article

Modulation of Temoporfin Distribution in Blood by β-Cyclodextrin Nanoshuttles

1
Centre de Recherche en Automatique de Nancy, Centre National de la Recherche Scientifique, UMR 7039, Université de Lorraine, Campus Sciences, Boulevard des Aiguillette, 54506 Vandoeuvre-lès-Nancy, France
2
Research Department, Institut de Cancérologie de Lorraine, 6 Avenue de Bourgogne, 54519 Vandoeuvre-lès-Nancy, France
3
Laboratory of Biophysics and Biotechnology, Faculty of Physics, Belarusian State University, 4 Nezavisimosti Avenue, 220030 Minsk, Belarus
4
Belarusian Research Center of Pediatric Oncology, Hematology, and Immunology, 223053 Minsk Region, Belarus
*
Author to whom correspondence should be addressed.
Academic Editors: Paola Mura and Marzia Cirri
Pharmaceutics 2021, 13(7), 1054; https://doi.org/10.3390/pharmaceutics13071054
Received: 8 June 2021 / Revised: 3 July 2021 / Accepted: 6 July 2021 / Published: 9 July 2021
(This article belongs to the Special Issue Cyclodextrins in Drug Delivery)
Photodynamic therapy represents a more targeted and less invasive alternative cancer treatment to traditional modalities. Temoporfin, as with many photosensitizers, is given by injection into a vein, and its subsequent fate is largely determined by the binding to plasma proteins and interaction with endothelial and blood cells. Thus, it is essential to be able to control and to alter the biodistribution of temoporfin in blood. In the present study, we evaluated the effect of co-administration of temoporfin with randomly methylated β-CD (Me-β-CD) on the distribution of temoporfin in the main subpopulations of blood cells of healthy donors using absorbance spectrophotometry and flow cytometry. We showed that cell-bound temoporfin fraction in blood strongly depends on the concentration of Me-β-CD. In fact, the accumulation of temoporfin in white blood cells was more sensitive than that in red blood cells, due to the higher volume of membranous organelles in white blood cells. Finally, we demonstrated that Me-β-CD significantly increases cellular uptake of temoporfin cancer human Burkitt′s lymphoma Raji cells. The presence of Me-β-CD resulted in a spotted pattern of temoporfin distribution in the plasma membrane compartment. Our results clearly demonstrated that β-CDs derivatives provide new options to modulate temoporfin biodistribution in blood. View Full-Text
Keywords: temoporfin; cyclodextrins; red blood cells; white blood cells; Raji human Burkitt’s lymphoma; flow cytometry temoporfin; cyclodextrins; red blood cells; white blood cells; Raji human Burkitt’s lymphoma; flow cytometry
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MDPI and ACS Style

Yakavets, I.; Yankovsky, I.; Zorina, T.; Belevtsev, M.; Bezdetnaya, L.; Zorin, V. Modulation of Temoporfin Distribution in Blood by β-Cyclodextrin Nanoshuttles. Pharmaceutics 2021, 13, 1054. https://doi.org/10.3390/pharmaceutics13071054

AMA Style

Yakavets I, Yankovsky I, Zorina T, Belevtsev M, Bezdetnaya L, Zorin V. Modulation of Temoporfin Distribution in Blood by β-Cyclodextrin Nanoshuttles. Pharmaceutics. 2021; 13(7):1054. https://doi.org/10.3390/pharmaceutics13071054

Chicago/Turabian Style

Yakavets, Ilya, Igor Yankovsky, Tatyana Zorina, Mikhail Belevtsev, Lina Bezdetnaya, and Vladimir Zorin. 2021. "Modulation of Temoporfin Distribution in Blood by β-Cyclodextrin Nanoshuttles" Pharmaceutics 13, no. 7: 1054. https://doi.org/10.3390/pharmaceutics13071054

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