Incorporation of Zinc into Binary SiO2-CaO Mesoporous Bioactive Glass Nanoparticles Enhances Anti-Inflammatory and Osteogenic Activities
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis and Ion Release Behavior of Zn-MBGs
2.2. Preparation of Extracts
2.3. Cell Culture
2.4. Cell Counting Kit-8 (CCK-8) Assay
2.5. Live/Dead Fluorescence Staining
2.6. Alizarin Red S Staining
2.7. Expression of Osteogenesis-Related Genes
2.8. Immunostaining of Osteocalcin (OCN)
2.9. Expression of Pro-Inflammatory Related Genes
2.10. iNOS Fluorescence Staining
2.11. Statistical Analysis
3. Results and Discussion
3.1. Ion Release of Zn-MBGs in Cell Culture Medium
3.2. In Vitro Cytotoxicity
3.3. In Vitro Cell Mineralization and Osteogenic Activities
3.4. Anti-Inflammatory Effects of Zn-MBGs
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Genes | Forward Primer Sequence | Reverse Primer Sequence |
---|---|---|
ALP | ATGCTCAGGACAGGATCAAA | CGGGACATAAGCGAGTTTCT |
RUNX2 | GGGACTGGTACTCGGACAAT | GGCCTTCTCATCCAGTTCAT |
COL-I | AGCTCGATACACAATGGCCT | CCTATGACTTCTGCGTCTGG |
OCN | CAGACAAGTCCCACACAGCA | CCAGCAGAGTGAGCAGAGAG |
β-actin | CCTCTATGACAACACAGT | AGCCACCAATCCACACAG |
Genes | Forward Primer Sequence | Reverse Primer Sequence |
---|---|---|
TNF-α | GCCTATGTCTCAGCCTCTT | GGTTGACTTTCTCCTGGTAT |
IL-6 | CGATAGTCAATTCCAGAAACCGC | TTGGGAGTGGTATCCTCTGTGAAG |
IL-1β | GCAACTGTTCCTGAACTCAACT | ATCTTTTGGGGTCCGTCAACT |
CD86 | TGTTTCCGTGGAGACGCAAG | TTGAGCCTTTGTAAATGGGCA |
GAPDH | GGACACTGAGCAAGAGAGGC | TTATGGGGGTCTGGGATGGA |
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Sun, H.; Zheng, K.; Zhou, T.; Boccaccini, A.R. Incorporation of Zinc into Binary SiO2-CaO Mesoporous Bioactive Glass Nanoparticles Enhances Anti-Inflammatory and Osteogenic Activities. Pharmaceutics 2021, 13, 2124. https://doi.org/10.3390/pharmaceutics13122124
Sun H, Zheng K, Zhou T, Boccaccini AR. Incorporation of Zinc into Binary SiO2-CaO Mesoporous Bioactive Glass Nanoparticles Enhances Anti-Inflammatory and Osteogenic Activities. Pharmaceutics. 2021; 13(12):2124. https://doi.org/10.3390/pharmaceutics13122124
Chicago/Turabian StyleSun, Haishui, Kai Zheng, Tian Zhou, and Aldo R. Boccaccini. 2021. "Incorporation of Zinc into Binary SiO2-CaO Mesoporous Bioactive Glass Nanoparticles Enhances Anti-Inflammatory and Osteogenic Activities" Pharmaceutics 13, no. 12: 2124. https://doi.org/10.3390/pharmaceutics13122124
APA StyleSun, H., Zheng, K., Zhou, T., & Boccaccini, A. R. (2021). Incorporation of Zinc into Binary SiO2-CaO Mesoporous Bioactive Glass Nanoparticles Enhances Anti-Inflammatory and Osteogenic Activities. Pharmaceutics, 13(12), 2124. https://doi.org/10.3390/pharmaceutics13122124