In Vivo Validation of Spray-Dried Mesoporous Bioactive Glass Microspheres Acting as Prolonged Local Release Systems for BMP-2 to Support Bone Regeneration

Julius-Wolff-Institut, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Charité–Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, 13353 Berlin, Germany

Berlin-Brandenburg School for Regenerative Therapies, Charité—Universitätsmedizin Berlin, 10117 Berlin, Germany

Department of Applied Science and Technology, Politecnico di Torino, 10129 Turin, Italy

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Institute of Biomaterials, University of Erlangen-Nuremberg, 91058 Erlangen, Germany

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BIH Center for Regenerative Therapies, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Charité–Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, 13353 Berlin, Germany

Author to whom correspondence should be addressed.

Pharmaceutics 2020, 12(9), 823; https://doi.org/10.3390/pharmaceutics12090823

Received: 4 August 2020 / Revised: 24 August 2020 / Accepted: 26 August 2020 / Published: 28 August 2020

(This article belongs to the Special Issue Implantable Materials for Drug Delivery in Tissue Regeneration)

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Abstract

Bone morphogenetic protein-2 (BMP-2) is a known key mediator of physiological bone regeneration and is clinically approved for selected musculoskeletal interventions. Yet, broad usage of this growth factor is impeded due to side effects that are majorly evoked by high dosages and burst release kinetics. In this study, mesoporous bioactive glass microspheres (MBGs), produced by an aerosol-assisted spray-drying scalable process, were loaded with BMP-2 resulting in prolonged, low-dose BMP-2 release without affecting the material characteristics. In vitro, MBGs were found to be cytocompatible and to induce a pro-osteogenic response in primary human mesenchymal stromal cells (MSCs). In a pre-clinical rodent model, BMP-2 loaded MBGs significantly enhanced bone formation and influenced the microarchitecture of newly formed bone. The MBG carriers alone performed equal to the untreated (empty) control in most parameters tested, while additionally exerting mild pro-angiogenic effects. Using MBGs as a biocompatible, pro-regenerative carrier for local and sustained low dose BMP-2 release could limit side effects, thus enabling a safer usage of BMP-2 as a potent pro-osteogenic growth factor. View Full-Text

Keywords: mesoporous bioactive glass; local drug release; BMP-2; fracture healing; autologous blood clot

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MDPI and ACS Style

Berkmann, J.C.; Herrera Martin, A.X.; Pontremoli, C.; Zheng, K.; Bucher, C.H.; Ellinghaus, A.; Boccaccini, A.R.; Fiorilli, S.; Vitale Brovarone, C.; Duda, G.N.; Schmidt-Bleek, K. In Vivo Validation of Spray-Dried Mesoporous Bioactive Glass Microspheres Acting as Prolonged Local Release Systems for BMP-2 to Support Bone Regeneration. Pharmaceutics 2020, 12, 823. https://doi.org/10.3390/pharmaceutics12090823

AMA Style

Berkmann JC, Herrera Martin AX, Pontremoli C, Zheng K, Bucher CH, Ellinghaus A, Boccaccini AR, Fiorilli S, Vitale Brovarone C, Duda GN, Schmidt-Bleek K. In Vivo Validation of Spray-Dried Mesoporous Bioactive Glass Microspheres Acting as Prolonged Local Release Systems for BMP-2 to Support Bone Regeneration. Pharmaceutics. 2020; 12(9):823. https://doi.org/10.3390/pharmaceutics12090823

Chicago/Turabian Style

Berkmann, Julia C., Aaron X. Herrera Martin, Carlotta Pontremoli, Kai Zheng, Christian H. Bucher, Agnes Ellinghaus, Aldo R. Boccaccini, Sonia Fiorilli, Chiara Vitale Brovarone, Georg N. Duda, and Katharina Schmidt-Bleek. 2020. "In Vivo Validation of Spray-Dried Mesoporous Bioactive Glass Microspheres Acting as Prolonged Local Release Systems for BMP-2 to Support Bone Regeneration" Pharmaceutics 12, no. 9: 823. https://doi.org/10.3390/pharmaceutics12090823

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In Vivo Validation of Spray-Dried Mesoporous Bioactive Glass Microspheres Acting as Prolonged Local Release Systems for BMP-2 to Support Bone Regeneration

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