Doxorubicin-Loaded PLGA Nanoparticles for Cancer Therapy: Molecular Weight Effect of PLGA in Doxorubicin Release for Controlling Immunogenic Cell Death
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of DOX-Loaded PLGA NPs
2.3. In Vitro Characterization of DOX-PLGA7K NPs and DOX-PLGA12K NPs
2.4. In Vitro Cellular Uptake, Cytotoxicity, and Immunogenic Cell Death Analysis
2.5. In Vivo Drug Release Analysis
2.6. Tumor Growth Inhibition and Mechanism Analysis
2.7. Tumor Re-Challenging and Interferon-Gamma (INF-γ) Detection
2.8. Statistical Analysis
3. Results and Discussions
3.1. Formulation and In Vitro Characterization of the DOX-PLGA7K NPs and DOX-PLGA12K NPs
3.2. In Vitro Cellular Uptake and Immunogenic Cell Death on the CT-26 Tumor Cells
3.3. In Vivo Drug Release and Tumor Growth Inhibition
3.4. Immune-Memory Effect Evaluation
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Choi, Y.; Yoon, H.Y.; Kim, J.; Yang, S.; Lee, J.; Choi, J.W.; Moon, Y.; Kim, J.; Lim, S.; Shim, M.K.; Jeon, S.; Kwon, I.C.; Kim, K. Doxorubicin-Loaded PLGA Nanoparticles for Cancer Therapy: Molecular Weight Effect of PLGA in Doxorubicin Release for Controlling Immunogenic Cell Death. Pharmaceutics 2020, 12, 1165. https://doi.org/10.3390/pharmaceutics12121165
Choi Y, Yoon HY, Kim J, Yang S, Lee J, Choi JW, Moon Y, Kim J, Lim S, Shim MK, Jeon S, Kwon IC, Kim K. Doxorubicin-Loaded PLGA Nanoparticles for Cancer Therapy: Molecular Weight Effect of PLGA in Doxorubicin Release for Controlling Immunogenic Cell Death. Pharmaceutics. 2020; 12(12):1165. https://doi.org/10.3390/pharmaceutics12121165
Chicago/Turabian StyleChoi, Yongwhan, Hong Yeol Yoon, Jeongrae Kim, Suah Yang, Jaewan Lee, Ji Woong Choi, Yujeong Moon, Jinseong Kim, Seungho Lim, Man Kyu Shim, Sangmin Jeon, Ick Chan Kwon, and Kwangmeyung Kim. 2020. "Doxorubicin-Loaded PLGA Nanoparticles for Cancer Therapy: Molecular Weight Effect of PLGA in Doxorubicin Release for Controlling Immunogenic Cell Death" Pharmaceutics 12, no. 12: 1165. https://doi.org/10.3390/pharmaceutics12121165