Next Article in Journal
PVP-H2O2 Complex as a New Stressor for the Accelerated Oxidation Study of Pharmaceutical Solids
Next Article in Special Issue
Ground Calcium Carbonate as a Low Cost and Biosafety Excipient for Solubility and Dissolution Improvement of Praziquantel
Previous Article in Journal
In Vitro Release of Bioactive Bone Morphogenetic Proteins (GDF5, BB-1, and BMP-2) from a PLGA Fiber-Reinforced, Brushite-Forming Calcium Phosphate Cement
Previous Article in Special Issue
Designing Fast-Dissolving Orodispersible Films of Amphotericin B for Oropharyngeal Candidiasis
Article

Increased Efficacy of Oral Fixed-Dose Combination of Amphotericin B and AHCC® Natural Adjuvant against Aspergillosis

1
Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili and Institut d’Investigació Sanitària Pere Virgili (IISPV), 43201 Reus, Tarragona, Spain
2
Departament of Pharmaceutics and Food Technology and Instituto Universitario de Farmacia Industrial (IUFI), School of Pharmacy, University Complutense, Avenida Complutense, 28040 Madrid, Spain
3
UCL School of Pharmacy, 29–39, Brunswick Square, London WC1N 1AX, UK
4
Nanomerics Ltd., St. Albans AL1 1SR, UK
*
Author to whom correspondence should be addressed.
Pharmaceutics 2019, 11(9), 456; https://doi.org/10.3390/pharmaceutics11090456
Received: 3 June 2019 / Revised: 17 August 2019 / Accepted: 19 August 2019 / Published: 3 September 2019
(This article belongs to the Special Issue Antifungal and Antiparasitic Drug Delivery)
Invasive pulmonary aspergillosis represents one of the most serious fungal infections among immunocompromised patients. In this study, we aimed to analyze the in vivo efficacy of prophylactic oral amphotericin B (AMB) encapsulated in modified chitosan-nanoparticles (Nanomerics’ Molecular Envelope Technology (MET)) supplemented with a standardized extract of cultured Lentinula edodes mycelia (AHCC®) in a murine model of pulmonary aspergillosis. We determined fungal burden and survival of mice and additionally, we carried out a cytokine analysis in an attempt to understand the immunomodulation of the extract. Our results evidenced equivalent efficacy between orally administered AMB-MET and the intravenous liposomal AMB marketed formulation. Addition of the AHCC® supplement significantly improved efficacy in terms of burden reduction and survival increase of both oral and intravenous AMB therapies compared to the untreated control group. Moreover, a protective effect of the extract was observed in terms of weight loss. Regarding the cytokine profiles, the Th1 immune response was stimulated in treated animals when compared to the control group. This response was marked by an enhancement in the MCP-1, GM-CSF, VEGF, RANTES and IL-17 levels and a decrease in the IL-6, a biomarker related to the severity of the infection. View Full-Text
Keywords: aspergillosis; amphotericin B; oral delivery; chitosan; shiitake; Lentinula edodes; AHCC®; Molecular Envelope Technology aspergillosis; amphotericin B; oral delivery; chitosan; shiitake; Lentinula edodes; AHCC®; Molecular Envelope Technology
Show Figures

Figure 1

MDPI and ACS Style

Pérez-Cantero, A.; Serrano, D.R.; Navarro-Rodríguez, P.; Schätzlein, A.G.; Uchegbu, I.F.; Torrado, J.J.; Capilla, J. Increased Efficacy of Oral Fixed-Dose Combination of Amphotericin B and AHCC® Natural Adjuvant against Aspergillosis. Pharmaceutics 2019, 11, 456. https://doi.org/10.3390/pharmaceutics11090456

AMA Style

Pérez-Cantero A, Serrano DR, Navarro-Rodríguez P, Schätzlein AG, Uchegbu IF, Torrado JJ, Capilla J. Increased Efficacy of Oral Fixed-Dose Combination of Amphotericin B and AHCC® Natural Adjuvant against Aspergillosis. Pharmaceutics. 2019; 11(9):456. https://doi.org/10.3390/pharmaceutics11090456

Chicago/Turabian Style

Pérez-Cantero, Alba, Dolores R. Serrano, Patricia Navarro-Rodríguez, Andreas G. Schätzlein, Ijeoma F. Uchegbu, Juan J. Torrado, and Javier Capilla. 2019. "Increased Efficacy of Oral Fixed-Dose Combination of Amphotericin B and AHCC® Natural Adjuvant against Aspergillosis" Pharmaceutics 11, no. 9: 456. https://doi.org/10.3390/pharmaceutics11090456

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop