Fabrication of Ion-Crosslinking Aminochitosan Nanoparticles for Encapsulation and Slow Release of Curcumin
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of Aminated Chitosan (AmCS)
2.3. Preparation of Folate-Modified Aminated Chitosan Sodium Tripolyphosphate (FA-AmCS-TPP) Nanoparticles
2.4. Characterization
2.4.1. Particle Size, Polydispersity Index (PDI), and Zeta (ζ)-Potential Measurement
2.4.2. Determination of FA
2.4.3. Drug-Loading Studies
2.4.4. In Vitro Release Studies
2.4.5. Cytotoxicity and Uptake by Tumor Cells
2.4.6. Other Characterization
3. Results and Discussion
3.1. Analysis of Shape and Appearance
3.2. Fourier Transform Infrared Spectrophotometry (FT-IR)
3.3. Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC)
3.4. Particle Size, PDI, and ζ-Potential
3.5. Factors Influencing Cur Encapsulation Efficiency (EE-Cur) and FA Encapsulation Efficiency (EE-FA)
3.5.1. The Effect of Drug Addition Method
3.5.2. The Effect of Drug Dosage
3.5.3. The Effect of Material Concentrations
3.6. In Vitro Release Studies of Cur/FA-AmCS-TPP Nanoparticles
3.7. Cytotoxicity and Uptake by Tumor Cells
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Samples | AmCS (g) | FA (mg) | TPP (mg) |
---|---|---|---|
S1 | 0.05 | 10 | 10 |
S2 | 0.10 | 10 | 10 |
S3 | 0.15 | 10 | 10 |
S4 | 0.20 | 10 | 10 |
S5 | 0.10 | 0 | 10 |
S6 | 0.10 | 5 | 10 |
S7 | 0.10 | 15 | 10 |
S8 | 0.10 | 10 | 5 |
S9 | 0.10 | 10 | 15 |
S10 | 0.10 | 10 | 20 |
Groups | Cur (mg) | EE-Cur (%) | |
---|---|---|---|
FA = 5 mg | FA = 10 mg | ||
1 | 2 | 73.99 ± 0.84 | 64.30 ± 1.65 |
2 | 5 | 90.06 ± 0.93 | 88.23 ± 0.25 |
3 | 8 | 93.34 ± 0.14 | 89.79 ± 0.64 |
4 | 10 | 94.25 ± 0.68 | 91.36 ± 1.21 |
5 | 15 | 97.02 ± 0.65 | 95.82 ± 0.94 |
6 | 30 | 98.10 ± 0.55 | 97.71 ± 0.84 |
7 | 35 | 91.68 ± 1.11 | 90.25 ± 1.08 |
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Sun, X.; Yu, D.; Ying, Z.; Pan, C.; Wang, N.; Huang, F.; Ling, J.; Ouyang, X.-k. Fabrication of Ion-Crosslinking Aminochitosan Nanoparticles for Encapsulation and Slow Release of Curcumin. Pharmaceutics 2019, 11, 584. https://doi.org/10.3390/pharmaceutics11110584
Sun X, Yu D, Ying Z, Pan C, Wang N, Huang F, Ling J, Ouyang X-k. Fabrication of Ion-Crosslinking Aminochitosan Nanoparticles for Encapsulation and Slow Release of Curcumin. Pharmaceutics. 2019; 11(11):584. https://doi.org/10.3390/pharmaceutics11110584
Chicago/Turabian StyleSun, Xiaoxiao, Dongyan Yu, Zhuoyang Ying, Chuqiao Pan, Nan Wang, Fangfang Huang, Junhong Ling, and Xiao-kun Ouyang. 2019. "Fabrication of Ion-Crosslinking Aminochitosan Nanoparticles for Encapsulation and Slow Release of Curcumin" Pharmaceutics 11, no. 11: 584. https://doi.org/10.3390/pharmaceutics11110584